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pLVET-HA-K-RasG12V-IRES-GFP

Plasmid

#107140

Purpose

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

HA-tagged Kras4B(G12V) and GFP (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hEF-1alpha

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-hSyn-EGFP

Plasmid

#114215

Purpose

Lentiviral vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9-Mxi1

Plasmid

#46921

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-Mxi1

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pKO403_lacZ_Sp

Plasmid

#174726

Purpose

Bifidobacterium-E.coli shuttle vector for seamless gene cloning

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

Type

Empty backbone

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CD44S pBabe-puro

Plasmid

#19127

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro-KrasG12D2B

Plasmid

#139786

Purpose

Lentiviral tet-inducible expression of KrasG12D (mouse)

Depositor

Joan Massague

Article

Su et al Nature. 2020 Jan;577(7791):566-571. doi:

Insert

KrasG12D (Kras Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

G12D

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Rheb

Plasmid

#133767

Purpose

Expresses GFP-tagged Rheb in mammalian cells

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

Rheb (RHEB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a URA3

Plasmid

#61924

Purpose

Expression of S. cerevisiae URA3 cloned into pFA6a

Depositor

Jon Houseley, David Tollervey

Article

Houseley et al Nucleic Acids Res. 2011 Nov 1;39(20):877

Insert

URA3

Use

Yeast genomic targeting

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRPC3-Myc

Plasmid

#25902

Depositor

Craig Montell

Article

Li et al Neuron. 1999 Sep . 24(1):261-73.

Insert

TRPC3 (Trpc3 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTNT-B18R

Plasmid

#58978

Purpose

expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSFV-Helper1

Plasmid

#92073

Purpose

This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.

Use

Helper plasmid, for run-off transcription and rna…

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP670nano

Plasmid

#127434

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

LifeAct-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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