176,063 results

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FLT3-ITD-MSCV-IRES-GFP

Plasmid

#184779

Purpose

Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

Depositor

Timothy Ley

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Activating internal tandem duplication (ITD) adde…

Promoter

MSCV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Publication

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plenti6-SLC7A11/xCT-V5

Plasmid

#170427

Purpose

Eepresses SLC7A11 (also named xCT) in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

Insert

solute carrier family 7 member 11 (SLC7A11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163056

Purpose

mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFP-KASH

Plasmid

#154373

Purpose

Vector for Cre-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG001 His6-TwinStrep-SUMO-AapCas12b

Plasmid

#153162

Purpose

Bacterial expression plasmid for AapCas12b

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

Insert

AapCas12b

Expression

Bacterial

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CMV-Citron1

Plasmid

#134303

Purpose

Mammalian expression of direct-response citrate sensor

Depositor

Robert Campbell

Publication

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citron1

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI152-pLenti-SiT-Cas12a

Plasmid

#128405

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Publication

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFUGW SpCas9

Plasmid

#131506

Purpose

lentiviral expression of SpCas9 under hUBC promotor

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Publication

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Publication

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-tTA

Plasmid

#104102

Purpose

Expresses tTA2 under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Publication

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Expression

Mammalian

Promoter

CAG

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCherry-CaaX(Hras)

Plasmid

#108886

Purpose

Expression of membrane localized mCherry

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry-CaaX(Hras)

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Publication

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Expression

Mammalian

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CKIIa-stGtACR2-FusionRed

Plasmid

#105669

Purpose

Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV Retrograde and AAV1

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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