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172,546 results

Showing: 6741 - 6760 of 172546 results

  9. pICE-HA-MRE11-H63S

    Plasmid
    #82035
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H63S MRE11 mutant
    Promoter
    CMV-tet
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82035-plasmid-map-sequence-id-155388

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  10. S1PR3-Tango

    Plasmid
    #66498
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    S1PR3 (S1PR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  13. RhoA sensor (LSS-SGFP2-mCherry)

    Plasmid
    #112948
    Purpose
    RhoA FRET biosensor
    Depositor
    Dorus Gadella
    Insert
    Rhoa sensor
    Tags
    LSS-SGFP2 and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112948-plasmid-map-sequence-id-220190

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  15. pQCXIP-cathepsinD-YMH

    Plasmid
    #182644
    Purpose
    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    cathepsinD
    Use
    Retroviral
    Tags
    His6-tag, TS site from proCCK, and myc-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182644-plasmid-map-sequence-id-358628

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Showing: 6741 - 6760 of 172546 results