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pEGFP-N1-CaMKKbeta S129D, S133D, S137D

Plasmid

#32455

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129D, S133D, S137D

Promoter

CMV

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only
Sema3g.a(L)-AP-His

Plasmid

#72025

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mouseAtoh1-IRES-GFP

Plasmid

#73572

Purpose

Mammalian retroviral expression of Atoh1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Promoter

MSCV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pBD-0072

Plasmid

#12361

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP E578Q (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

E578Q

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only
Anti-KChIP3/DREAM [K66/38R]

Plasmid

#128625

Purpose

Mammalian Expression Plasmid of anti-KChIP3/DREAM (Rat). Derived from hybridoma K66/38.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-KChIP3/DREAM (Rat) recombinant mouse monoclonal antibody (Kcnip3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-MOR4-1

Plasmid

#22329

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR4-1 (Olfr620 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-mFndc3b

Plasmid

#126443

Purpose

Expresses mouse Fndc3b circular RNA

Depositor

Jeremy Wilusz

Article

Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

Insert

Fndc3b (Fndc3b Mouse)

Expression

Mammalian

Mutation

circFndc3b exons are separated by a miniature int…

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

Plasmid

#126444

Purpose

Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

Depositor

Jeremy Wilusz

Article

Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

Insert

Fndc3b (Fndc3b Mouse)

Expression

Mammalian

Mutation

circFndc3b exons are separated by a miniature int…

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPN1zetawt.15

Plasmid

#109291

Purpose

Express TCR zeta-GFP fusion protein

Depositor

Nicolai van Oers

Insert

TCR zeta (Cd247 Mouse)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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Ncam1_1.0.1-AP-His

Plasmid

#71961

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.0.1 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-222-PGK-GFP-Blast

Plasmid

#31522

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-222 (Mir222 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
pGL4.24-Cd36-enhancer5

Plasmid

#138577

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17893352_17894696

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe 3xFLAG mouse Bmf

Plasmid

#17243

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Article

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

Plasmid

#51249

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 980,1006 mutated to Ala

Promoter

EF1a

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only
mVenus-proα2(I)

Plasmid

#119838

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7 shRNA against CRTC1

Plasmid

#72710

Purpose

Lentiviral expression of shRNA against CRTC1 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC1

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-ELK3

Plasmid

#98622

Purpose

Gateway entry TOPO TA cloning vector for ELK3

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK3 (Elk3 Mouse)

Use

Topo ta cloning vector

Mutation

E167K

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-mUCH37 (ISF2)

Plasmid

#61928

Purpose

Expression of mUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

UCH37 (M.musculus) (Uchl5 Mouse)

Tags

6x HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-Fc-His

Plasmid

#72142

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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