We narrowed to 1,429 results for: tk

1. pLN472 (FuGW-G8p-STE-1Pe)

   Plasmid

   #105190

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-STE-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMRX-IP/Venus-mATG5

   Plasmid

   #58739

   Purpose

   Expresses Venus-mATG5 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3 14 mut B-Myb

   Plasmid

   #25967

   Depositor

   Rob Lewis

   Article

   Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

   Insert

   B-Myb (Mybl2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Changed threonines 267, 408, 443, 447 490, 497, 5…

   Promoter

   Available Since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pOP461

   Plasmid

   #85934

   Purpose

   Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

   Depositor

   Timothy Lu

   Article

   Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

   Insert

   Anti-Ebola 4G7 monoclonal antibody
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLN397 (FuGW-S(E2F1)p-Sponge7)

   Plasmid

   #105181

   Purpose

   Module 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(E2F1)p-Sponge7
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

   Plasmid

   #105182

   Purpose

   Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   S(E2F1)p-ECFP (Cerulean) sponge control
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMRX-IP/SECFP-mATG5

   Plasmid

   #58744

   Purpose

   Expresses /SECFP-ATG5 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   SECFP

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLV-CAG-F14F15S-BFP2-(mCherry)'

   Plasmid

   #115235

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Flp recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMM1162

   Plasmid

   #112535

   Purpose

   pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107 and ProD

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRVdG-4FLPo

    Plasmid

    #122050

    Purpose

    Genome plasmid for deletion-mutant rabies virus encoding Flpo recombinase

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

    Plasmid

    #138368

    Purpose

    Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLN553 (FuGW-S(MAFK)p-Sponge7)

    Plasmid

    #105203

    Purpose

    Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(MAFK)p-Sponge7
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLD-CAG-DYSF-CPL

    Plasmid

    #131458

    Purpose

    Encodes Dysferlin protein (Therapeutic gene) protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    DYSF (DYSF Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRV∆G-N*PEST-4Cre

    Plasmid

    #174381

    Purpose

    Rabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domain

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human MAC-1 alpha

    Plasmid

    #8631

    Depositor

    Timothy Springer

    Article

    Corbi et al J Biol Chem 1988 Sep 5;263(25):12403-11.

    Insert

    MAC-1 alpha (ITGAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAG43

    Plasmid

    #226736

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SpySwitch

    Plasmid

    #184225

    Purpose

    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpySwitch
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E77A mutation prevents isopeptide bond formation,…

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid

    #55201

    Purpose

    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Accessory plasmid 1

    Plasmid

    #128569

    Purpose

    for mixing with SPECS library to produce lentivirus

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Insert

    ECFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGM227

    Plasmid

    #220182

    Purpose

    Expresses exogenous wild-type TOMM70A

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TOMM70A (TOMM70 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGM117

    Plasmid

    #220168

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    CISD1 (CISD1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid

    #55200

    Purpose

    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMM627

    Plasmid

    #112532

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107, PL(HrtO), and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRX-IP/SECFP-hATG16A1

    Plasmid

    #58994

    Purpose

    Expresses SECFP-hATG16A1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3.1-Flag-H2A K118-119R

    Plasmid

    #63564

    Purpose

    Expression of human H2A with K118R and K119R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 118 and Lysine 119 are now Arginine

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid

    #105200

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRX-IP/Venus-rLC3

    Plasmid

    #58740

    Purpose

    Expresses Venus-rLC3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3.1-Flag-H2A K13-15R

    Plasmid

    #63563

    Purpose

    Expression of human H2A with K13R and K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 13 and Lysine 15 are now Arginine

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only