We narrowed to 75,668 results for: Rest
-
Plasmid#17417DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pACP-tag (m)- 2 Vector
Plasmid#101126PurposeMammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of ACP-tag.DepositorTypeEmpty backboneUseTagsACP-tagExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCP-tag (m) Vector
Plasmid#101127PurposeMammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.DepositorTypeEmpty backboneUseTagsMCP-tag (Mutant ACP- tag)ExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
-
-
pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXP420 v2 (repaired Amp promoter)
Plasmid#86920PurposeShuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
p404MET3
Plasmid#17419DepositorInsertMET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
371-C
Plasmid#97029PurposeHis6-MBP-N10-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, MBP domain, N10 linker, and TEV cleavag…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pQC V5 MCS IRES G418
Plasmid#110345Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromotertetR/pLtetOAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p406MET3
Plasmid#17436DepositorInsertMET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET3 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
p406GALL
Plasmid#17434DepositorInsertGALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALL promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
-
pHT-497
Plasmid#87756PurposeBacterial expression vector for producing 6x histidine tagged rat DYRK1A kinase domain (residues 1-497)DepositorInsertGene coding for rat DYRK1A kinase domain (residues 1-497)
UseTags6X histidine tagExpressionBacterialMutationPromoterT7 RNA polymerase promoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p404GALL
Plasmid#17418DepositorInsertGALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALL promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
p159-RV-syn-dio-mScarlet-T2A
Plasmid#185700PurposeRetroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)DepositorInsertmScarlet
UseRetroviralTagsmScarlet palmitoylation with T2A on 3'-endExpressionMutationPromoterSynapsinAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-GFP1-9
Plasmid#121684PurposePPI reporterDepositorInsertGFP1-9
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GFP1-9
Plasmid#113613PurposePPI reporterDepositorInsertGFP1-9
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
-
pQLinkHD
Plasmid#13668DepositorInsertGateway cassette
UseCo-expressionTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pQLinkGD
Plasmid#13673DepositorInsertGateway cassette
UseCo-expressionTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
XLone-eGFP NLS (no drug resistant gene)
Plasmid#140032PurposeTunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
IMBB2.4-pUC57-mini
Plasmid#88845Purposeplasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)DepositorTypeEmpty backboneUseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
p404GALS
Plasmid#17435DepositorInsertGALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALS promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHT-PRD
Plasmid#87755PurposeBacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)DepositorInsertSemi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
UseTags6X histidine tagExpressionBacterialMutationPromoterT7 RNA polymerase promoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-PS
Plasmid#86628PurposeExpresses Akt pseudosubstrate tagged with mCherryDepositorInsertAkt pseudosubstrate (VELDPEFEPRARERAYAFGH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHaloTag-EGFP
Plasmid#86629PurposeExpresses HaloTag-eGFP tandem (cytosolic)DepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EGFP
Plasmid#86639PurposeExpresses mCherry-eGFP tandem (cytoplasmic)DepositorInsertmCherry-EGFP
UseTagsmCherry-EGFPExpressionMammalianMutationPromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUltra-H2B-mTagBFP2
Plasmid#161791Purpose3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; MulticistronicTagsEGFP and mTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbCAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
T11-HA-hORF1
Plasmid#185053Purposeexpression of human L1 ORF1 proteinDepositorInsertL1-hORF1
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-HA
Plasmid#119756PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGDestSC+ATG
Plasmid#49323PurposeDestination vector for Golden Gate assembly; Few restriction sites for subcloning.DepositorTypeEmpty backboneUseDestination vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMS2-1
Plasmid#220627PurposePlasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHaloTag-PS
Plasmid#86626PurposeExpresses Akt pseudosubstrate tagged with a HaloTagDepositorInsertHaloTag-PS(RA)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmRFPmars
Plasmid#26252DepositorInsertmRFPmars
UseTags6xHisExpressionBacterialMutationKpnI restriction site removedPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUltra-hot-H2B-mTagBFP2
Plasmid#161792Purpose3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interestDepositorInsertH2B-mTagBFP2
UseLentiviral; MulticistronicTagsmCherry and mTagBFP2ExpressionMammalianMutationI174A in the mTagBFP2 protein according to PLoS O…PromoterUbCAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p404MET25
Plasmid#17420DepositorInsertMET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationMET25 promoter is between SacI and XbaI restricti…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
13SA-csy4
Plasmid#97037PurposeSpectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectorsDepositorInsertCys4
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only