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Showing: 1651 - 1700 of 79968 results

  3. pBabe-Puro-BRAF-V600E

    Plasmid
    #15269
    Purpose
    Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
    Depositor
    William Hahn
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Insert
    BRAF (BRAF Human)
    Use
    Retroviral
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Valine 600 to Glutamate (mutation has been found …
    Available Since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15269-plasmid-map-sequence-id-217979

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  4. pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #108274
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    EF1α promoter (1.1kb short version)
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108274-plasmid-map-sequence-id-262430

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  5. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

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  6. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

    Plasmid
    #228902
    Purpose
    For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD
    Depositor
    Bryan Roth
    Article
    Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.
    Has Service
    AAV8
    Insert
    hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
    Use
    AAV and Synthetic Biology
    Tags
    HA and P2A mCitrine
    Expression
    Mammalian
    Mutation
    R108K
    Promoter
    Chicken Beta Actin
    Available Since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228902-plasmid-map-sequence-id-454100

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  15. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

    Plasmid
    #172907
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has Service
    AAV1
    Insert
    Voltron2-ST
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    172907-plasmid-map-sequence-id-353194

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  16. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid
    #172908
    Purpose
    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has Service
    AAV1
    Insert
    Ace2N-4AA-mNeon-ST A122D
    Use
    AAV and Cre/Lox
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    hSynapsin1
    Available Since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172908-plasmid-map-sequence-id-341477

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  17. AV0hSynWS

    Plasmid
    #124966
    Purpose
    Express gene of interest in neurons
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    AAV
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    124966-plasmid-map-sequence-id-243873

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  18. pHIV-dTomato

    Plasmid
    #21374
    Purpose
    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato
    Depositor
    Bryan Welm
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    21374-plasmid-map-sequence-id-292244

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  19. pCDH-EF1

    Plasmid
    #72266
    Purpose
    Express gene of interest under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72266-plasmid-map-sequence-id-447561

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  20. pJPN0018

    Plasmid
    #112083
    Purpose
    eCFP module CT
    Depositor
    Christian Chevalier
    Insert
    eCFP
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112083-plasmid-map-sequence-id-218022

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  21. pJPN0016

    Plasmid
    #112077
    Purpose
    tagRFP module CT
    Depositor
    Christian Chevalier
    Insert
    TagRFP
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112077-plasmid-map-sequence-id-217643

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  22. pUltra-hot

    Plasmid
    #24130
    Purpose
    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    Feb. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    24130-plasmid-map-sequence-id-247629

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  23. pCDH-CB

    Plasmid
    #72267
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72267-plasmid-map-sequence-id-131747

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  24. AV0-BWS-2.0

    Plasmid
    #124976
    Purpose
    Express gene of interest under EF1 promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    AAV
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124976-plasmid-map-sequence-id-243968

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  25. pJPN0003

    Plasmid
    #112080
    Purpose
    GFP module NT2
    Depositor
    Christian Chevalier
    Insert
    GFP
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112080-plasmid-map-sequence-id-217645

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  26. pJPN0015

    Plasmid
    #112081
    Purpose
    tagRFP module NT2
    Depositor
    Christian Chevalier
    Insert
    TagRFP
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112081-plasmid-map-sequence-id-217647

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  27. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72268-plasmid-map-sequence-id-134573

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  28. AV0-hSyn-SV40pA

    Plasmid
    #124965
    Purpose
    Express gene of interest under human Syn promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    AAV
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    124965-plasmid-map-sequence-id-243708

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  29. pORFE0011

    Plasmid
    #112075
    Purpose
    pcUBI promoter module Pro+5U
    Depositor
    Christian Chevalier
    Insert
    pcUBI promoter
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112075-plasmid-map-sequence-id-217625

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  30. TY5_hisAI_2micron

    Plasmid
    #138622
    Purpose
    Ty5 restrotransposon with a reversed artificial intron used for Calling Cards experiment
    Depositor
    Rob Mitra
    Insert
    TY5
    Expression
    Yeast
    Promoter
    Gal1_10
    Available Since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138622-plasmid-map-sequence-id-334952

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  31. pJPN0017

    Plasmid
    #112082
    Purpose
    eCFP module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    eCFP without stop codon
    Expression
    Plant
    Mutation
    BsaI and BpiI restriction sites have been remove…
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112082-plasmid-map-sequence-id-217656

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  32. pHalo2X-N3

    Plasmid
    #251420
    Purpose
    Mammalian expression vector for expressing a protein of interest with a C-terminal HaloTag
    Depositor
    Thomas Friedman, Takushi Miyoshi
    Type
    Empty backbone
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Two silent mustations to disable the XhoI and SalI
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251420-plasmid-map-sequence-id-501387

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  33. pUltra-Smurf

    Plasmid
    #48974
    Purpose
    3rd generation Lentiviral vector with bi-cistronic expression of the blue fluorescent protein AmCyan and the gene of interest.
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    AmCyan
    Expression
    Mammalian
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    48974-plasmid-map-sequence-id-255027

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  34. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRI
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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  35. pOpen-EcoRV

    Plasmid
    #165548
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRV
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165548-plasmid-map-sequence-id-339428

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  36. pOpen-SapIR

    Plasmid
    #165537
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SapI
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165537-plasmid-map-sequence-id-339127

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  37. pCS2+mScarlet3-C Cloning Vector

    Plasmid
    #220616
    Purpose
    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    mScarlet3 fusion
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220616-plasmid-map-sequence-id-435943

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  38. pRS414-Syf1

    Plasmid
    #234114
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS414 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234114-plasmid-map-sequence-id-465994

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  39. pOpen-XbaIR

    Plasmid
    #165567
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    XbaI
    Use
    Synthetic Biology
    Available Since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165567-plasmid-map-sequence-id-340099

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  40. pOpen-PstIR

    Plasmid
    #165526
    Purpose
    Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    PstI
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165526-plasmid-map-sequence-id-339060

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  41. pOpen-HindIIIR

    Plasmid
    #165508
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    HindIII
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165508-plasmid-map-sequence-id-338587

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  42. pOpen-NcoIR

    Plasmid
    #165568
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    NcoI
    Use
    Synthetic Biology
    Available Since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165568-plasmid-map-sequence-id-340100

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  43. pRS416-Syf1

    Plasmid
    #234113
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS416 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234113-plasmid-map-sequence-id-466460

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  44. pUltra-Plus

    Plasmid
    #161775
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
    Depositor
    Jorge Luis Arias-Arias
    Type
    Empty backbone
    Use
    Lentiviral; Multicistronic
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161775-plasmid-map-sequence-id-316822

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  45. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Eco31I
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165506-plasmid-map-sequence-id-338512

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  46. pRS414-Fyv6-CFLAG

    Plasmid
    #234133
    Purpose
    Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234133-plasmid-map-sequence-id-466523

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  47. pRS414-NFLAG-Fyv6

    Plasmid
    #234139
    Purpose
    Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234139-plasmid-map-sequence-id-466518

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  48. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway Cloning
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

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  49. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway Cloning
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

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  50. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

    Enlarge

Showing: 1651 - 1700 of 79968 results