19,660 results

1. pCMVTNT-T7-KPNA1

   Plasmid

   #26677

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 1 (KPNA1 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   KPNA1 sequence contains a reported SNP at S73N

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1436 pcDNA3 Flag HA

   Plasmid

   #10792

   Purpose

   pcDNA3 vector with added Flag, HA tags

   Depositor

   William Sellers

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHM840

   Plasmid

   #20701

   Depositor

   Thomas Stamminger

   Article

   Sorg et al Biotechniques. 1999 May . 26(5):858-62.

   Insert

   GFP-NLS-lacZ
   

   Expression

   Mammalian

   Available Since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. cfSGFP2-anti-AlfaTag nanobody

   Plasmid

   #171818

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   cfSGFP2-anti-AlfaTag nanobody
   

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPCX roGFP2-Orp1

   Plasmid

   #64991

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-HA-DUB3

   Plasmid

   #22593

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   DUB3 (USP17L9P Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-IRAlus-Nicn1

   Plasmid

   #92345

   Purpose

   Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHel3

   Plasmid

   #102961

   Purpose

   E.coli/H.Pylori shuttle vector

   Depositor

   Rainer Haas

   Article

   Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

   Type

   Empty backbone

   Use

   Shuttle vector

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Mutation

   D908A

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PRK5-FLAG-Bag6ΔUBL

    Plasmid

    #61837

    Purpose

    express N-terminally FLAG tagged human Bag6 without UBL domain

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-NES-PhoCl2f-mCherry

    Plasmid

    #164048

    Purpose

    Optogenetic control of protein localization by PhoCl2f

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2f-mCherry
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-RNaseHI-NES-EGFP

    Plasmid

    #196701

    Purpose

    Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    bacterial RNaseHI
    

    Use

    Lentiviral

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-zTRAP

    Plasmid

    #42236

    Depositor

    Stephen L Johnson

    Article

    Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

    Insert

    EGFP-Rpl10a (rpl10a Zebrafish)
    

    Tags

    EGFP

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR SFFVp Phy-mCherry-CAAX

    Plasmid

    #50839

    Purpose

    Optogenetic PI3K activation system; membrane component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Insert

    PhyB 1-908
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-NSP3SA11

    Plasmid

    #89170

    Purpose

    Express Rotavirus SA11 NSP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP3
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28HIS-hAPE1

    Plasmid

    #70757

    Purpose

    bacterial expression of human APE1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    APEX1 (APEX1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRIPZ-bioGEF-UBwt-PURO

    Plasmid

    #208045

    Purpose

    Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    Ub (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pR BirA

    Plasmid

    #113640

    Purpose

    Insect cell expression vector for BirA Biotin Ligase

    Depositor

    James Moon

    Article

    Moon et al Methods Mol Biol. 2018;1799:165-181. doi

    Insert

    BirA Biotin Ligase (birA )
    

    Expression

    Insect

    Promoter

    Metallothionein

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tubulin-mGreenLantern

    Plasmid

    #164460

    Purpose

    Localization of mGreenLantern fluorescent protein to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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