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mCerulean3-Dectin1A-N-10
Plasmid#55414PurposeLocalization: Membrane, Excitation: 433, Emission: 475DepositorInsertDectin1A (CLEC7A Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_zyxin
Plasmid#65683PurposeExpresses Zyxin-ONL in mammalian cellsDepositorInsertzyxin (Zyx Mouse)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-gRNA_zebrafish_mmp21
Plasmid#72890Purposeplasmid to generate guideRNA against mmp21 gene in zebrafishDepositorInsertzebrafish mmp21 (mmp21 Zebrafish)
UseCRISPRTagsExpressionMutationPromoterT7 RNA polAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_TUBB5
Plasmid#65680PurposeExpresses beta-tubulin-ONL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Dectin1A-C-10
Plasmid#55413PurposeLocalization: Membrane, Excitation: 433, Emission: 475DepositorInsertDectin1A (CLEC7A Human)
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL1855
Plasmid#53041PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.pTYB1.a21-281&390-428.L239R
Plasmid#15959DepositorInsertEscherichia coli surA delta PPIase domain 2
UseTagsinteinExpressionBacterialMutationresidues 282-389 deleted; L239R mutationPromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGST1-GGA2-VHS
Plasmid#44419DepositorInsertGGA2-VHS (GGA2 Human)
UseTagsGSTExpressionBacterialMutationAlanine 23 changed to ThreoninePromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-RFP-SIKE
Plasmid#235691PurposeExpress RFP tagged SIKE gene in mammalian cells using the CMV promoterDepositorInsertSIKE (SIKE1 Human)
UseLentiviralTagsRFPExpressionMammalianMutationPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-GTSE1
Plasmid#224719PurposeFor expression of HA-tagged GTSE1 proteinDepositorInsertGTSE1 (GTSE1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 Luc2-IRES-GFP
Plasmid#224467PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFPDepositorInsertLuciferase
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS∆P2
Plasmid#225197PurposeUsed to create a markerless deletion of the E. coli HS P2 phageDepositorInsertP2 phage homology arms
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3235
Plasmid#234107PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertsfGFP
UseTagsFLAGExpressionBacterialMutationS30R/Y39N/N105T/Y145F/I171V/A206VPromoterpJ23100-BsaIAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3220
Plasmid#234099PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertsfGFP
UseTagsFLAGExpressionBacterialMutationS30R/Y39N/N105T/Y145F/I171V/A206VPromoterpJ23100-BsaIAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3237
Plasmid#234091PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertsfGFP
UseTagsFLAGExpressionBacterialMutationS30R/Y39N/N105T/Y145F/I171V/A206VPromoterpJ23100-BsaIAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1821
Plasmid#228327PurposeConstitutive mutant rTetTA expressionDepositorInsertrtetTA mutant
UseSynthetic BiologyTagsExpressionYeastMutationK8NPromoterScPGK1 promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1375
Plasmid#228314PurposeConstitutive expression of mutant rTetTADepositorInsertrtetTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)ExpressionYeastMutationK8NPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3400
Plasmid#234084PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertsfGFP
UseTagsFLAGExpressionBacterialMutationS30R/Y39N/N105T/Y145F/I171V/A206VPromoterpJ23100-BsaIAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4402
Plasmid#215253PurposeModule for the expression of the N. nambi HISPS gene under the regulation of PMTB and TNOS and the constitutive expression of CPH,LUZ, H3H, EGFP and p19DepositorInsertSF-PMTB:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Impact-T4gp61(I342G)
Plasmid#228208PurposeExpression in BL21(DE3) of T4 primase I342GDepositorInsert61 (61 Escherichia phage T4)
UseTagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated isoleucine 342 to glycine (I342G), improv…PromoterT7Available sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-YR-ICUE-mVenus
Plasmid#231787PurposeVisualization of cAMP dynamics using YR-ICUE-mVenusDepositorInsertYR-ICUE-mVenus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-YR-ICUE-mGold
Plasmid#231788PurposeVisualization of cAMP dynamics using YR-ICUE-mGoldDepositorInsertYR-ICUE-mGold
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
ECMAB - pET22b Ag43 160N 6H Mms6
Plasmid#225539PurposeConstruction of extracellular magnetite accumulating bacterial systems.DepositorInsertsIron binding protein
Autotransporter protein
UseTagsnoExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb
Plasmid#214951PurposeBacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusionsDepositorInsertsVNp-anti-Lysozyme Fab heavy chain fusion
VNp-anti-Lysozyme Fab light chain fusion
UseTagsVNpExpressionBacterialMutationPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430-G-G mm
Plasmid#221728PurposeExpression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.DepositorInsertBleoR-miR-430-G-G mm
UseFish expressionTagsExpressionMutationG-G mismatch of miR-430 sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Zeo-miR-430-10-11 mm
Plasmid#221727PurposeExpression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.DepositorInsertBleoR-miR-430-10-11 mm
UseFish expressionTagsExpressionMutationMismatch at positions 10 and 11 of miR-430 sitePromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sasgRNACMV- mCherry-WPREpA
Plasmid#217015PurposeS. aureus sgRNAs backbone along with mCherry expressionDepositorInsertmCherry
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
SBC015898
Plasmid#226285PurposeExpresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.DepositorInsertsConiferyl alcohol acyltransferase
Eugenol synthase 1
Eugenol O-methyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only
