We narrowed to 13,600 results for: sequence
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Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
HRE-doUnaG
Plasmid#201713PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequenceDepositorInsertUnaG
ExpressionMammalianAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSC-GFP-NES-EGFP
Plasmid#221810PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).DepositorInsertGFP-NES-EGFP
UseLentiviralTagsNES (internal tag)ExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-RpHLuorin2
Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
TagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^Halo
Plasmid#174108PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.DepositorInsertCodon optimized VPS13D
TagsHaloExpressionMammalianMutationWith an internal Halo tag in aminoacid position 1…PromoterCMVAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
TagsC-terminal FLAG tag with a linkerExpressionBacterialAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
Plasmid#50022Purposeexpresses Membrane tagged G-CaMP3 in cells expressing CreDepositorInsertG-CaMP3
UseAAVTagsMARCKS sequence (MGCCFSKT)PromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB
Plasmid#228869PurposePE7 connected to MLH1-SB via 2A sequenceDepositorInsertPE7-2A-MLH1-SB
UseCRISPRAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBigT
Plasmid#21270PurposeUsed for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.DepositorTypeEmpty backboneUseMouse TargetingAvailable SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS
Plasmid#158058PurposeDonor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable markerDepositorInsertsglmS homologous region 1
glmS homologous region 2 + MCS3
UseSynthetic BiologyAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENV8(WT)-GFPuv
Plasmid#70127PurposeCobalamin riboswitch reporter vector for use in E. coliDepositorInsertwild type environmental cobalamin riboswitch sequence (env8)
UseSynthetic BiologyTagsNoneExpressionBacterialPromotertacAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-mGarnet2
Plasmid#104310Purposecodes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mitoDepositorInsertmito-mGarnet2
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterU6Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
TagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianPromoterEF-1aAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHBS1147 GFP-TDP43
Plasmid#118797PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP (TARDBP Human)
UseLentiviralAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only