We narrowed to 26,401 results for: Vit

1. pcDNA3.1-mas-KB-1753-Nluc

   Plasmid

   #158603

   Purpose

   Plasmid expressing KB-1753-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCMV4a-SIRT6-H133Y-FLAG

   Plasmid

   #102324

   Purpose

   Human SIRT6 H133Y with FLAG tag for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR221_SLC22A1

   Plasmid

   #132034

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. RCAS-K-ras

   Plasmid

   #11549

   Depositor

   Sandra Orsulic

   Article

   Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

   Insert

   v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)
   

   Use

   Retroviral

   Mutation

   G12D

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pTagBFP-Akt1

   Plasmid

   #86594

   Purpose

   Expresses TagBFP-tagged human Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Nuc-ExRai-AMPKAR

   Plasmid

   #192450

   Purpose

   ExRai-AMPKAR targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR-NLS
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Mito-CaNAR2

   Plasmid

   #64731

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Tags

   Cerulean3, N-terminal 30 amino acids from DAKAP1,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. N1-mSin1.2-GFP

   Plasmid

   #72908

   Purpose

   GFP-tagged mSin1 (isoform 2)

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+ Gal4-GBP2-IRES-GBP7-p65

    Plasmid

    #50020

    Purpose

    Multi-purpose expression of a GFP-dependent transcription factor

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
    

    Use

    Multi-purpose expression

    Promoter

    CMV-immediate early promoter and SP6 promoter

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSV-CaMKII

    Plasmid

    #45064

    Purpose

    Expression of CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVETL-Cmcs-Flag-Ataxin3Q80

    Plasmid

    #22120

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-DAKAP-μDpTT

    Plasmid

    #182324

    Purpose

    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DAKAP-μDronpa-TagRFP-T
    

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-sh-mSOX9-2

    Plasmid

    #40645

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-2 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-mCherry-Rheb(5A)

    Plasmid

    #192439

    Purpose

    Encodes mCherry-tagged Rheb lacking residues 38-42.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb amino acids 38-42 deleted.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVETL-Cmcs-Flag-Ataxin3Q25

    Plasmid

    #22118

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-GBP1-10gly-Gal4DBD

    Plasmid

    #49438

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-Gal4 DNA binding domain fusion protein
    

    Expression

    Mammalian

    Mutation

    C98S (to increase protein solubility), Q3D

    Promoter

    CAG

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET22b_EcSSB_1

    Plasmid

    #78203

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

    Insert

    ssb (G26C)
    

    Expression

    Bacterial

    Mutation

    G26C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p1071-Lenti-Single loxP

    Plasmid

    #217892

    Purpose

    Lentiviral vector, entry vector for Lentiviral Switch-OVER

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge