19,779 results

Showing: 10441 - 10470 of 19779 results

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sox10:mCherry

Plasmid

#98695

Purpose

I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

mCherry

Use

Zebrafish expression

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pST-KT

Plasmid

#44561

Purpose

for ectopic inducible expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Promoter

UV15 promoter

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pMJ920

Plasmid

#42234

Depositor

Jennifer Doudna

Article

Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

Insert

Cas9

Use

CRISPR

Tags

GFP, HA epitope, and NLS (nuclear localization si…

Expression

Mammalian

Mutation

codon-optimized synthetic DNA sequence

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-AURKB

Plasmid

#23682

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKB (AURKB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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mAzurite-C1

Plasmid

#54583

Purpose

Localization: C1 Cloning Vector, Excitation: 384, Emission: 450

Depositor

Patrick Daugherty, Michael Davidson

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Type

Empty backbone

Tags

mAzurite

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pC018 - LshCas13a from Leptotrichia shahii

Plasmid

#91908

Purpose

Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LshCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available Since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMyBADC-kan

Plasmid

#84689

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

AraC

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Tuba

Plasmid

#22214

Depositor

Pietro De Camilli

Article

Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

Insert

Tuba (DNMBP Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
eTC GFP beta-actin full length

Plasmid

#27123

Depositor

Robert Singer

Article

Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

Insert

beta-actin (ACTB Human)

Tags

EGFP and TC tag

Expression

Mammalian

Mutation

E237A

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

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PM-FRB-Cerulean-T2A-FKBP-5-ptase

Plasmid

#40897

Depositor

Peter Varnai

Article

Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

Insert

PM-FRB-Cerulean-T2A-FKBP-5-ptase

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA PTEN

Plasmid

#78776

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRc/RSV-m CBP-HA

Plasmid

#16701

Depositor

Richard Goodman

Article

Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

Insert

CBP (Crebbp Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_RNAseH1

Plasmid

#65784

Purpose

expresses GFP-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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PB-CA-rtTA Adv

Plasmid

#20910

Purpose

piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

rtTA-ON-Adv

Use

piggybac

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-At-TRL

Plasmid

#32242

Depositor

Stan Fields, Jay Hesselberth

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Tags

6xHistidine

Expression

Bacterial

Promoter

T7 RNA Polmyerase

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE4398

Plasmid

#74042

Purpose

Expresses human codon-optimized LbCpf1 and Lb crRNA.

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Lb crRNA
LbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK3

Plasmid

#23509

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK3 (MAPK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAG423GAL-ccdB

Plasmid

#14149

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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Nuclear Myosin I YFP

Plasmid

#60241

Purpose

Expresses nuclear isoform of myosin IC attached to YFP and an NLS

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-REL

Plasmid

#27253

Depositor

Thomas Gilmore

Article

Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

Insert

REL (REL Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-WT

Plasmid

#45699

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
IL-2 promoter luciferase

Plasmid

#12194

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-2 promoter (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge

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19,779 results