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We narrowed to 2,087 results for: Endog

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Showing: 1701 - 1750 of 2087 results

  4. pSP569

    Plasmid
    #139499
    Purpose
    Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pSTL1 24xPP7sl STL1 100 - 600
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pSTL1
    Available Since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139499-plasmid-map-sequence-id-292017

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  8. pJK174

    Plasmid
    #71835
    Purpose
    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7 and endogenous
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71835-plasmid-map-sequence-id-131052

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  10. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-487542

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  11. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Promoter
    human PGK1
    Available Since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    68375-plasmid-map-sequence-id-251912

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  12. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid
    #202568
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202568-plasmid-map-sequence-id-429225

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  13. pAAV-S5E2-FingR-PSD95-mGL

    Plasmid
    #217533
    Purpose
    Genetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivo
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    ZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Promoter
    S5E2 enhancer
    Available Since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217533-plasmid-map-sequence-id-429605

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  24. pAC-EHER

    Plasmid
    #53262
    Purpose
    Contains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, idi, crtX, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53262-plasmid-map-sequence-id-82988

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  27. pAC-85b

    Plasmid
    #53282
    Purpose
    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, crtY, crtI
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53282-plasmid-map-sequence-id-82995

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  35. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  44. GTH-ir

    Plasmid
    #81107
    Purpose
    Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    81107-plasmid-map-sequence-id-166610

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  46. GTH-y

    Plasmid
    #81105
    Purpose
    Gene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    mCitrine
    Use
    Cre/Lox
    Tags
    mCitrine
    Expression
    Yeast
    Mutation
    Mutated mCitrine for monomeric isoform (A206K L22…
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81105-plasmid-map-sequence-id-154304

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  48. pONSY

    Plasmid
    #111873
    Purpose
    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Type
    Empty backbone
    Use
    Capsaspora owczarzaki
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111873-plasmid-map-sequence-id-217304

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  49. miR-144 Trap

    Plasmid
    #60934
    Purpose
    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter
    Depositor
    Curt Civin
    Article
    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111
    Insert
    miR-144 Trap
    Use
    Lentiviral
    Tags
    GFP
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. GTU-g

    Plasmid
    #81094
    Purpose
    Gene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81094-plasmid-map-sequence-id-154249

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Showing: 1701 - 1750 of 2087 results