Addgene

We narrowed to 9,510 results for: Gnas

Showing: 1701 - 1750 of 9510 results

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pEM705 Gsz

Plasmid

#109355

Purpose

GsX chimera with Gαz C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsz chimera

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-ROCK2

Plasmid

#225472

Purpose

Express mEGFP-fusion protein of ROCK2

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

ROCK2 (ROCK2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Spop

Plasmid

#128872

Purpose

For mammalian expression of mouse Spop

Depositor

Aimin Liu

Article

Cai et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Spop (Spop Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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hPGK-rtTA3

Plasmid

#195883

Purpose

Lentiviral construct of hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

rtTA3

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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EphA1-RTK-Cry2olig

Plasmid

#176601

Purpose

EphrinA1 RTK fusion to Cry2olig - RTK domain only

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

EphA1rtk-Cry2olig-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCBFRE-luc

Plasmid

#26897

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only
pEBG-Pak1 83-149

Plasmid

#12214

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180590

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Tags

Expression

Mutation

PHR(E281A)

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRR-MR-5Z

Plasmid

#23059

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

MR (NR3C2 Human)

Use

Tags

Expression

Yeast

Mutation

N528H, Q814R, H993L

Promoter

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pT7-pelB

Plasmid

#157740

Purpose

empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Use

Tags

PelB signal sequence and possible His6-tag (adds …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pME turquoise2

Plasmid

#135207

Purpose

Cyan fluorescent tag

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

mTurquoise2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASx alpha

Plasmid

#15209

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx alpha (PIAS2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-PRKAA1

Plasmid

#30305

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 (Prkaa1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2 deltaB deltaM

Plasmid

#16069

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Vx3K0-mEGFP

Plasmid

#35527

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3K0

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3puro-mGFP-TOP3B

Plasmid

#225470

Purpose

Express mEGFP-fusion protein of TOP3B

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

TOP3B (TOP3B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-Vx3NB-mEGFP

Plasmid

#35528

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3NB-mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR Fyn-TagBFP

Plasmid

#171035

Purpose

TagBFP-tagged Fyn kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Fyn

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T-IQGAP1

Plasmid

#30107

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5myc POSH

Plasmid

#15908

Depositor

Alan Hall

Article

Tapon et al EMBO J. 1998 Mar 2. 17(5):1395-404.

Insert

POSH (Sh3rf1 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pPAP004

Plasmid

#160911

Purpose

E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

lacZ selection cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ11440

Plasmid

#183203

Purpose

pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

hyperdCas12 base editor

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR Lck-TagBFP

Plasmid

#171034

Purpose

TagBFP-tagged Lck kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Lck

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YF-Rac1(CA)

Plasmid

#20150

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(CA) (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

constitutively active mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pME VIVIT NS

Plasmid

#188699

Purpose

Gateway middle element encoding the VIVIT peptide (no stop codon)

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VIVIT

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB-p53shRNA

Plasmid

#28222

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

p53 shRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA4TO-24xGCN4_v1-P2A-sfGFP

Plasmid

#61057

Purpose

sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v1 and sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pECFP-Rac1Q61L

Plasmid

#105292

Purpose

Constitutively active Rac 1

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Rac1 (RAC1 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 hSmad1-MM

Plasmid

#22990

Depositor

Edward De Robertis

Article

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-PRKAA2

Plasmid

#30310

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α2 (Prkaa1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS926

Plasmid

#123052

Purpose

LexA bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexA-NLS [3a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-mULK1M92A

Plasmid

#31964

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER.PKCdelta.RNAi

Plasmid

#10819

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC delta RNAi (PRKCD Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11429

Plasmid

#183205

Purpose

pHR-R(NFKB-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ23101

Plasmid

#70252

Purpose

Expresses YFP from J23101 promoter and CFP from standard J23101 promoter

Depositor

Jim Haseloff

Article

Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

Insert

promoter J23101-RBS B0034-EYFP-B0015

Use

Tags

Expression

Mutation

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-Rx3(A7)-mEGFP

Plasmid

#35531

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3(A7)-mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pTet-ctl

Plasmid

#136693

Purpose

Control for pTet-Gli2shR

Depositor

LuZhe Sun

Article

Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-tVYN-TmΔ

Plasmid

#149694

Purpose

High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-TmYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Neo HA-hSki

Plasmid

#10910

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

Ski (SKI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER.PKCepsilon.RNAi

Plasmid

#10798

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC epsilon RNAi (PRKCE Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2006

Availability

Academic Institutions and Nonprofits only
CMVTO-SEAP-26Sfur-3TM-tevs-AAMP

Plasmid

#179633

Purpose

SEAP RELEASE construct with AAMP motif control

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ11445

Plasmid

#183629

Purpose

pHR-R(ARE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-C1-mCherry-LOVPLD

Plasmid

#219559

Purpose

ER-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

C1-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM705 Gs13

Plasmid

#109359

Purpose

GsX chimera with Gα13 C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gs13 chimera

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)_CMVtrunc_877

Plasmid

#105828

Purpose

A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1701 - 1750 of 9510 results

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