We narrowed to 7,452 results for: Lif;
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Plasmid#172535PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistanceDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL_dstβL
Plasmid#73805PurposePlasmid for selection for insertion and homodimerizationDepositorInsertsCAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain
UseTagsPart Of the dstbl chiemra, Part of the dsTbL chim…ExpressionBacterialMutationPromoterNaN and ToxR promotorAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8m
Plasmid#172388PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8m
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-erHRP(N175S mutant)
Plasmid#79909PurposeerHRP(N175S mutant) pCMV backboneDepositorInserterHRP(N175S mutant)
UseTagsHA tagExpressionMammalianMutationN175S mutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-5-SV40 polyA
Plasmid#190869PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode5
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-Kv-P2A-H2b-mRuby2
Plasmid#89570PurposeChannelrhodopsin localized to neuronal soma with simultaneous nuclear labelingDepositorInsertsChannelrhodopsin
Histone 2B
UseAAVTagsmRuby2ExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH3626
Plasmid#179630PurposePacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsRFPExpressionWormMutationPromoterPacr-2(s)Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBXNPH3
Plasmid#110098PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)
Plasmid#193853PurposePromoterless insertion vector for split HygR/mScarlet-I landing padDepositorInsert5' rps-0::HygR + 5' mScarlet-I
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterrps-0pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
C204
Plasmid#174358PurposeFor expressing siRNA-resistant human PABPC1 in human cell lines via transfectionDepositorInsertPABPC1 (PABPC1 Human)
UseTagsFLAGExpressionMammalianMutationMultiple silent mutations introduced to be resist…PromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.mCherry-FlpO
Plasmid#172378PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized mCherry + FlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-AKAR4
Plasmid#64733PurposeEndoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.DepositorInsertAKAR4
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Ac-AChBP
Plasmid#63128PurposeExpression of Aplysia californica AChBPDepositorInsertAChBP
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT-SEP
Plasmid#187653PurposeContains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.DepositorInsertHaloTag-SEP donor
UseAAV and CRISPRTagsN/AExpressionMutationPromoterN/AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
P447-FBP_19
Plasmid#139710PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsiveDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
SAC1C389SdeltaTMD-FKBP-mCherry
Plasmid#108124PurposeRecruitable cytosolic inactive SAC1DepositorInsertSACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(C389S; 1-520)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
P445-FBP_17
Plasmid#139708PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlledDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPI226
Plasmid#113228Purposesafe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherryDepositorInsertLifeAct
UseSafe haven knock-in plasmid for the act5 locus, l…TagsmCherryExpressionMutationPromoteract5Available sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL001_Hec1-ms_IgG_HC
Plasmid#193618PurposeHec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.DepositorInsertAnti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
UseTagsHis6 and signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL002_Hec1-ms_IgG_LC
Plasmid#193619PurposeHec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.DepositorInsertAnti-Hec1 light chain (Hec1-ms_IgG_LC)
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXs-Pou4f3
Plasmid#158530PurposeExpresses human Pou4f3 in mammalian cellsDepositorInsertPOU Class 4 Homeobox 3 (POU4F3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS5
Plasmid#111763PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
UseTagsGFPExpressionBacterial and YeastMutationPromoterSpunHsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ntn4-AP-His
Plasmid#71980PurposeExpresses the entire Netrin 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtn4 (Ntn4 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai
Plasmid#202568PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-FlpO
Plasmid#172375PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + FlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPI227
Plasmid#113231Purposesafe haven knock-in plasmid for the act5 locus, Expression of LifeAct-GFPDepositorInsertLifeAct
UseSafe haven knock-in plasmid for the act5 locus,li…TagsGFPExpressionMutationPromoteract5Available sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-STX17TMΔC
Plasmid#221737PurposeStable expression of GFP-STX17TMΔC in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-228 and 227-302PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PP-HaloTag-SYT7alpha
Plasmid#179714PurposeLentiviral expression of mouse Syt7 with N terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD
Plasmid#179720PurposeLentiviral expression of plasma membrane-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-tomato-TSC2-sgRNA
Plasmid#196195PurposeEditing human TSC2 locusDepositorInsertTSC2 (TSC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
UseTagsGFP and hphExpressionBacterial and YeastMutationPromoterSpun Hsp70prAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE
Plasmid#136916PurposeExpresses ChR2(H134R)-EYFP in Cre-negative cellsDepositorInsertChR2(H134R)-EYFP
UseAAVTagsExpressionMutationH134R in ChR2PromoterHuman Synapsin1Available sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Mito-CaNARi
Plasmid#64851PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNARi
UseTagsCerulean, N-terminal 30 amino acids from DAKAP1, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2 sgSp1
Plasmid#174907PurposeSecond generation sgRNA against Sp1DepositorInsertSpecificity Protein 1 (SP1 Human)
UseTagsExpressionMammalianMutationMissense mutation to mutate PAM sequencePromoterEFS-NSAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'S-mito
Plasmid#158765PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMVtight-RFP-IRES-CD
Plasmid#126678PurposeDoxycycline inducible expression of CD along with reporter RFPDepositorInsertRFP-IRES-CD (FCY1 Budding Yeast)
UseTagsExpressionMutationPromotertight TREAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68 R443A
Plasmid#162652PurposeExpresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationAmino acid R443 mutated to alaninePromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-tdTomato-ChIEF
Plasmid#172370PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInserttdTomato + ChIEF
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Plasmid#125225PurposeExpresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cellsDepositorInsertgLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T VPS4B, residues 1-86
Plasmid#180639PurposeBacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.DepositorInsertVPS4B (VPS4B Human)
UseTagsGSTExpressionBacterialMutationResidues 1-86PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW-lox-mCherry-hyBRET-ERK-NLS
Plasmid#170290PurposeFor generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.DepositorInsertlox-mCherry-lox-hyBRET-ERK-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Dead
Plasmid#173073PurposeDead SNAP tag at the centrosomeDepositorInsertmCherry-SNAP-SNAP-Varied-PACT
UseTagsmCherry-SNAP-SNAP-VariedExpressionMammalianMutationAA144 in both SNAP tags changed from a cysteine t…PromoterCMV IE94Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
All-in-one split-ADAR2
Plasmid#170136PurposeAAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signalDepositorInsertADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Cas9-POLD3
Plasmid#183197PurposeEntry cloneDepositorInsertCas9-POLD3
UseCRISPR; Gateway entry cloneTagsExpressionMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only