We narrowed to 6,070 results for: nls

1. pCW57-mcGAS-LL/RK-NLS

   Plasmid

   #185457

   Purpose

   Inducible nuclear cGAS

   Depositor

   Shitao Li

   Article

   Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

   Insert

   cGAS
   

   Use

   Lentiviral

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   L157R, L159K

   Promoter

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RANLS-LEHD-BNES

   Plasmid

   #60973

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Use

   Tags

   Caspase9 substrate sequence (LEHD) is after NLS s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

3. FnoCas12a-2C-NLS in pCSDest

   Plasmid

   #126639

   Purpose

   Expresses FnoCas12a-2C-NLS in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME tdTomato-NLS

   Plasmid

   #135205

   Purpose

   Red fluorescent tag

   Depositor

   David Tobin

   Insert

   tdTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREP3-TEV-NLS

   Plasmid

   #21146

   Depositor

   Stephen Kearsey

   Article

   Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

   Insert

   TEV protease (TEVgp1 tobacco etch virus)
   

   Use

   S. pombe

   Tags

   2xNLS, NLS, and myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. RANLS-IETD-BNES

   Plasmid

   #60972

   Purpose

   Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A and ddRFP B
   

   Use

   Tags

   NES sequence LALKLAGLDIGS and triplicated NLS seq…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-NLS-ZapCmR2

   Plasmid

   #59012

   Purpose

   Nuclear zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NLS-ZapCmR2 genetically encoded Zn(II) sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. PhyB-mCherry-NLS

   Plasmid

   #51571

   Purpose

   Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   NLS and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid

    #178236

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.VSVg.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid

    #178234

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.S.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCitrine-PCNA-19-SV40NLS-4

    Plasmid

    #56564

    Purpose

    Localization: Nucleus, Excitation: 516, Emission: 529

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pILL5-NLS-3xmCitrine

    Plasmid

    #192525

    Purpose

    Fluorescent reporter for plant responses to bacterial infection

    Depositor

    Gitta Coaker

    Article

    Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

    Insert

    IAA-leu-resistant-like5 (ILL5 Mustard Weed)
    

    Use

    Tags

    3xmCitrine and NLS

    Expression

    Plant

    Mutation

    Promoter

    ILL5

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME nls-eos

    Plasmid

    #73791

    Purpose

    Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

    Depositor

    David Raible

    Article

    Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

    Insert

    nls-eos
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. mVenus-PCNA-19-SV40NLS-4

    Plasmid

    #56621

    Purpose

    Localization: Nucleus, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    PCNA-19 (PCNA Human)
    

    Use

    Tags

    3xSV40NLS and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47454

    Purpose

    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    CRY2PHR-NLS-SID4X
    

    Use

    AAV

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2A-EKAREV-NLS

    Plasmid

    #173856

    Purpose

    Encoding EKAREV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKAREV-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-NPM NLS2D

    Plasmid

    #13285

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa150-aa155

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pQC NLS mKorange IX

    Plasmid

    #37346

    Depositor

    Connie Cepko

    Article

    Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

    Insert

    NLS mKusabira Orange
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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