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Apple-53BP1trunc

Plasmid

#69531

Purpose

Fluorescent reporter for DNA damage

Depositor

Ralph Weissleder

Publication

Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

Apple fluorescent protein

Expression

Mammalian

Promoter

CMV

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pET-15b-EK_C122S_His5

Plasmid

#49048

Purpose

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

Depositor

Hans Brandstetter

Publication

Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

Insert

Enteropeptidase, peptidase domain (TMPRSS15 Bovine)

Tags

5xHis

Expression

Bacterial

Mutation

P92R, E185D, C122S

Promoter

T7

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.3_SOX2

Plasmid

#26817

Depositor

Derrick Rossi

Publication

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-SOX2-3'UTR (SOX2 Human)

Expression

Mammalian

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles

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pCAG-NLS-HA-Bxb1

Plasmid

#51271

Purpose

Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

Depositor

Pawel Pelczar

Publication

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Bxb1

Expression

Mammalian

Promoter

CAG

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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mCerulean N1

Plasmid

#27795

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

mCerulean N1

Expression

Mammalian

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Publication

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pRS300

Plasmid

#22846

Purpose

Backbone for expressing plant artificial miRNAs

Depositor

Detlef Weigel

Publication

Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

Insert

MIR319a (MIR319a Mustard Weed)

Use

RNAi; Plant gene inactivation

Expression

Plant

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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phOCT4-Luc

Plasmid

#17221

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

Upstream region of human OCT4 (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCMV5B-Flag-Smad3

Plasmid

#11742

Depositor

Jeff Wrana

Publication

Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

Insert

SMAD3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

16 Articles

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pCMV-flag YAP2 5SA

Plasmid

#27371

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP2 (YAP1 Human)

Tags

flag

Expression

Mammalian

Mutation

Serines at positions 61, 109, 127, 128, 131, 163,…

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

51 Articles

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Publication

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pCAGGS-flpE-puro

Plasmid

#20733

Purpose

Mammalian expression of FLPe recombinase from the highly-expressed CAGGS promoter; puro selectable marker

Depositor

Rudolf Jaenisch

Publication

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

FLPe recombinase

Use

Flp/frt

Expression

Mammalian

Promoter

CAGGS

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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pet30b-7M SrtA

Plasmid

#51141

Purpose

expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Insert

S.aureus SrtA 7M

Tags

6x His

Expression

Bacterial

Mutation

P94R, E105K, E108Q, D160N, D165A, K190E, K196T

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pBiFC-bFosVC155

Plasmid

#22013

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosVC155

Tags

HA

Expression

Mammalian

Mutation

N/A

Available since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Publication

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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PCS2+Cas9-mSA

Plasmid

#103882

Purpose

Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

CRISPR

Tags

MSA (monomeric streptavidin

Mutation

none

Promoter

SP6

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMV-HA-UTX

Plasmid

#24168

Purpose

Mammalian expression of human UTX with HA tag

Depositor

Kristian Helin

Publication

Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Insert

UTX (KDM6A Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pUC57-mitoLbNOX

Plasmid

#74448

Purpose

This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

Depositor

Vamsi Mootha

Publication

Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

Insert

mitoLbNOX

Tags

Flag tag with linker and mitochondrial targeting …

Mutation

Human codon optimized

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pRRLsin-SV40 T antigen-IRES-mCherry

Plasmid

#58993

Purpose

lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

Depositor

Snorri Thorgeirsson

Publication

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein

Use

Lentiviral

Expression

Mammalian

Mutation

EcoRV site in MCS converted to BamHI site

Promoter

CMV enhancer-chicken beta actin

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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RelA cFlag pcDNA3

Plasmid

#20012

Depositor

Stephen Smale

Publication

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

RelA (Rela Mouse)

Tags

Flag

Expression

Mammalian

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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