171,827 results

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Article

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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pNC-Cam3304-RNAi

Plasmid

#193401

Purpose

Nimble cloning-compatible vector for plant RNAi

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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OPEN Beta2-microglobulin

Plasmid

#215657

Purpose

E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN Beta2-microglobulin (B2M Human)

Expression

Bacterial

Mutation

Histidine 31 to Cysteine

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCk3_spacer

Plasmid

#136073

Purpose

Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-BEC enhancer-minBG-Cre

Plasmid

#236724

Purpose

Cre Brain Endothelial Cell Enhancer

Depositor

Xiangmin Xu

Article

Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

Inserts

cis-regulatory elements 89763
Cre Recombinase

Use

AAV

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1b

Plasmid

#63136

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1b

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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nLbCas12a

Plasmid

#213745

Purpose

For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

nLbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

IRES-EGFP

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-Renilla

Plasmid

#87065

Purpose

Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Tags

Renilla luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-Bip-GFP:V5:KDEL

Plasmid

#69917

Purpose

An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

Depositor

Kenneth Irvine

Article

Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

Insert

GFP

Tags

Drosophila BiP signal sequence, KDEL ER targeting…

Expression

Insect

Promoter

pMT

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Article

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX_TRE3G_DD::mCherry

Plasmid

#108679

Purpose

Tunable system for controlled gene expression and protein stability in mammalian cells

Depositor

Lucia Marucci

Article

Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

Insert

Destabilizing Domain (DD) mCherry fusion protein

Use

Lentiviral

Tags

DD::mCherry fusion protein

Expression

Mammalian

Promoter

Doxycycline inducible

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGM1202

Plasmid

#69615

Purpose

inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3_038

Plasmid

#123752

Purpose

Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

R-FlincA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pME-Cas9

Plasmid

#63154

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9

Use

CRISPR; Tol2 middle entry vector for gateway

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CRF1.0

Plasmid

#208661

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC30A7_STOP

Plasmid

#161314

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A7 (SLC30A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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a1Ic-HE3-pcDNA3

Plasmid

#45810

Depositor

Edward Perez-Reyes

Article

Gomora et al Biophys J. 2002 Jul;83(1):229-41.

Insert

CACNA1I (CACNA1I Human)

Use

Making crna for oocyte injections

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A3

Plasmid

#135505

Purpose

Mammalian expression of myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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GRM1-DuET

Plasmid

#213300

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GRM1 (GRM1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJG103

Plasmid

#158533

Purpose

wrmScarlet11 x3 tandem repeats

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet11 x3 tandem repeats

Expression

Bacterial

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-GRAM-H

Plasmid

#211700

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (EGFP-GRAM1b G187L) in mammalian cells

Depositor

Yasunori Saheki

Article

Ercan et al EMBO J. 2021 Mar 15;40(6):e106524. doi:

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Glycine 187 to Leucine

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1s-ST-WPRE

Plasmid

#214968

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostability

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1s-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDM030

Plasmid

#216810

Purpose

Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Type

Empty backbone

Use

CRISPR; Fungal expression

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-FLAG-mCherry-C1-centrin-1

Plasmid

#73333

Purpose

Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

FLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pWJ68

Plasmid

#46571

Purpose

Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
w-dcas9
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

Plasmid

#162037

Purpose

Background-free mRNA labeling tripartite superfolder GFP system

Depositor

Hye Yoon Park

Article

Park et al RNA. 2020 Jan;26(1):101-109. doi: 10.126

Insert

GFP1-9 P2A MCP-GFP10 P2A PCP-GFP11

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1_AOX-FLAG

Plasmid

#177984

Purpose

All-in-one dox-inducible expression of Alternative Oxidase

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

AOX

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Codon optimized cDNA for expression in human cells

Promoter

TRE

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

Plasmid

#224435

Purpose

AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mScarlet-KASH
U6-gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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