We narrowed to 26,401 results for: Vit

1. pCMV-LOx-IRES-EGFP

   Plasmid

   #166877

   Purpose

   Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

   Depositor

   Sergey Kasparov

   Article

   Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

   Insert

   Lactate oxidase
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_tKiMBI-T2A-caMEK

   Plasmid

   #199578

   Purpose

   Expresses tKiMBI and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
   constitutively active MEK
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-BE4-PpAPOBEC1 H122A

   Plasmid

   #138338

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1H122A

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1 H122A
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Mutation

   Change of His122 to Ala.

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

   Plasmid

   #47387

   Purpose

   allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   glutamate-gated chloride Channels
   

   Expression

   Mammalian

   Mutation

   Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-Basic-hCYP26A1P-E4-mCherry

   Plasmid

   #135478

   Purpose

   Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   mCherry Open reading Frame
   

   Use

   Unspecified

   Promoter

   Short from promoter of human CYP26A1 (312 bp)

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAFNF-ECFP-Actb

   Plasmid

   #169787

   Purpose

   An actin FRET sensor. Must be expressed with Venus-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRRL-5xTetO-pEF-H2B-Citrine-SV40

   Plasmid

   #186966

   Purpose

   Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   5xTetO-pEF-H2B-Citrine-SV40
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   5xTetO-pEF-1alpha

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP95

   Plasmid

   #83550

   Purpose

   Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PDA1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   PDA1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-Lyn-μDpTT

   Plasmid

   #182321

   Purpose

   Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lyn-μDronpa-TagRFP-T
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CYP450-μDpTT

    Plasmid

    #182325

    Purpose

    Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DAKAP-μDronpa-TagRFP-T
    

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lifeact-μDpTT

    Plasmid

    #182322

    Purpose

    Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-μDronpa-TagRFP-T
    

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SG3ΔENV CA E28A,E29A

    Plasmid

    #145785

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residues Glutamic Acid residues 28…

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLAG-ING4

    Plasmid

    #13291

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING4 (ING4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLKO.1-sh-mSlug-3

    Plasmid

    #40647

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-3 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pHR-FRB:Akt2

    Plasmid

    #72899

    Purpose

    Recruitable Akt2 (LocaTOR2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Serine/threonine protein kinase Bβ (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEG(R1-R4) #BGV057

    Plasmid

    #48957

    Purpose

    Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. hLOXL2-S300P

    Plasmid

    #200061

    Purpose

    LOXL2 mutant

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Expression

    Mammalian

    Mutation

    S300P mutation

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEG R3-miRNA(ccdB)-L4 #BGV228

    Plasmid

    #48953

    Purpose

    To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only