We narrowed to 17,385 results for: puromycin
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Plasmid#130886PurposeExpresses human RAB10 K154A mutant with N-terminal GFP tagDepositorAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only
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Mouse 5' Npm1 AID GFP PuroR
Plasmid#127899PurposeHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Npm1 GeneDepositorAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgACSL3.C-Cas9-P2A-Puro
Plasmid#129412PurposeEncoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminusDepositorInsertSpCas9 and sgACSL3.C (ACSL3 Human)
UseCRISPRTagsP2A-PuroExpressionMammalianPromoterhU6Available SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-Q61L-shortlinker
Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.Available SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-P29S-shortlinker
Plasmid#128583PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Proline29 to Serine in the RAC1 moiety.Available SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PHB-4
Plasmid#122231PurposeExpresses shRNA targeting the 3' UTR of human PHBDepositorAvailable SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-PHB-8
Plasmid#122233PurposeExpresses shRNA targeting the coding sequence of human PHBDepositorInsertshRNA targeting PHB (see partial depositor seq) (PHB1 Human)
UseLentiviral and RNAiExpressionMammalianAvailable SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
LN-entry+NLS
Plasmid#128011PurposeVector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag-SV40NLS
UseLamdan entry vector with nls to generate tetherin…TagsLN-3xFlag-SV40NLSAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-CUL4A K705R-P2A-Puro
Plasmid#124305PurposeLentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoterDepositorInsertCUL4A (CUL4A Human)
UseLentiviralExpressionMammalianMutationchanged Lysine 705 to ArgininePromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-RNF110
Plasmid#126547PurposeExpresses GFP-tagged RNF110 in mammalian cellsDepositorAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shWRN2
Plasmid#125782Purposeconstitutive expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN2 (WRN Human)
UseRNAiAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-p52-HDM2_sensor
Plasmid#127184PurposeEukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interactionDepositorInsertp53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
TagsHisTag and StrepTagExpressionMammalianPromoterCMV-TetO2Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgWRN-EIJ
Plasmid#125774Purposeconstitutive expression of a guide RNA targeting an exon-intron junction of human WRNDepositorInsertsgWRN-EIJ (WRN Human)
UseCRISPRAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-E31A
Plasmid#125843Purposeexpress mutant BAP1-E31ADepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-S10T
Plasmid#125842Purposeexpress mutant BAP1-S10TDepositorAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA285= pSin-TCRab-CMVp-Puro
Plasmid#116875Purposestable TCR expression in human T cellsDepositorUseLentiviralExpressionMammalianMutationdeletion of EF1-a promotorPromoterCMV PromotorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
DKC1 F2.3 gRNA
Plasmid#90653Purpose3rd generation lentiviral gRNA plasmid targeting human DKC1DepositorInsertDKC1 (Guide Designation F2.3)
UseCRISPR and LentiviralPromoterU6Available SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116A
Plasmid#123245PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationGlycine 116 to AlaninePromoterPGKAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only