We narrowed to 13,271 results for: cDNA

1. pemiRFP703-tubulin

   Plasmid

   #136564

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   emiRFP703-tubulin
   

   Use

   Tags

   emiRFP703

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hHSF1 K80R

   Plasmid

   #118350

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to R.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80R

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcpBpaRS

   Plasmid

   #196484

   Purpose

   To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (synthetase), Pol III tRNA promoter (tRNA)

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

   Plasmid

   #128607

   Purpose

   Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   36xMashTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Opto-alpha1AR

   Plasmid

   #106064

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-alpha1AR
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-Halo-10-PFN1-G118V

   Plasmid

   #204225

   Purpose

   Expression of Halo-Profilin-1(G118V) in tissue culture cells

   Depositor

   Jessica Henty-Ridilla

   Article

   Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

   Insert

   PFN1 (PFN1 Human)
   

   Use

   Tags

   Halo, TEV site, SGLRSRAQAS linker

   Expression

   Mammalian

   Mutation

   G118V

   Promoter

   CMV

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. UKNP1.21.5

   Plasmid

   #98203

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NSP3-FR-2Strep

   Plasmid

   #210315

   Purpose

   Expresses SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP3 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Gal4DB-NS3-VP64-p65_IRES_mCherry

   Plasmid

   #112627

   Purpose

   Encodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

   Depositor

   John Ngo

   Article

   Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

   Insert

   Gal4DB-NS3-VP64-p65
   

   Use

   Tags

   AU1 (N term on NS3), HA (C term on NS3), and T7 (…

   Expression

   Mammalian

   Mutation

   NS3 mutant T54A

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. UKNP1.23.1

    Plasmid

    #98206

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

    Plasmid

    #169533

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. UKNP1.20.1

    Plasmid

    #98195

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAL175

    Plasmid

    #22276

    Purpose

    Expresses YFP-PIF for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ER-ABKAR

    Plasmid

    #61508

    Purpose

    Expression of AMPK biosensor targeted to the endoplasmic reticulum

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    ER targeting sequence from CYP450 2C1 (see commen…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. UKNP1.17.1

    Plasmid

    #98189

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UKNP1.20.3

    Plasmid

    #98197

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GreenT-EC.b - Display - CyRFP1

    Plasmid

    #193944

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-Halo-10-PFN1-Y6D

    Plasmid

    #204223

    Purpose

    Expression of Halo-Profilin-1(Y6D) in tissue culture cells

    Depositor

    Jessica Henty-Ridilla

    Article

    Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

    Insert

    PFN1 (PFN1 Human)
    

    Use

    Tags

    Halo, TEV site, SGLRSRAQAS linker

    Expression

    Mammalian

    Mutation

    Y6D

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-Halo-10-PFN1-R88E

    Plasmid

    #204224

    Purpose

    Expression of Halo-Profilin-1(R88E) in tissue culture cells

    Depositor

    Jessica Henty-Ridilla

    Article

    Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

    Insert

    PFN1 (PFN1 Human)
    

    Use

    Tags

    Halo, TEV site, SGLRSRAQAS linker

    Expression

    Mammalian

    Mutation

    R88E

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. μ2-HA-D176A

    Plasmid

    #32754

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D176A

    Promoter

    CMV

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

21. HA-merlin

    Plasmid

    #32836

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    Merlin (NF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pVH64-Tier1-PSV40-Citrine_p2a_SEAP

    Plasmid

    #169558

    Purpose

    Tier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PSV40-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PSV40

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pC3.1_CAG_oROS-Gr

    Plasmid

    #216113

    Purpose

    Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    CAG_oROS-Gr
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GreenT-EC.c - Display - CyRFP1

    Plasmid

    #193945

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. syntaxin3-myc-myc-His

    Plasmid

    #12372

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector

    Promoter

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-CBS (WT)

    Plasmid

    #182923

    Purpose

    Mammalian expression plasmid for human CBS

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PM-ABKAR

    Plasmid

    #61506

    Purpose

    Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    Lyn kinase sequence (see comments)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

28. Opto-D2R

    Plasmid

    #106068

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-D2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Equalizer-H plasmid

    Plasmid

    #169367

    Purpose

    Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Equalizer-M plasmid

    Plasmid

    #169731

    Purpose

    Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge