We narrowed to 28,416 results for: REP

1. GG-EBNA-OCT4

   Plasmid

   #69537

   Purpose

   Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   5 concatenated gRNA transcriptional cassettes targeting OCT4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2a-copGFP(+HA)

   Plasmid

   #83806

   Purpose

   Express copGFP reporter for HDR-induced knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   copGFP
   

   Use

   Homologous recombination for gapdh loci

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRT801

   Plasmid

   #68112

   Purpose

   Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.

   Depositor

   Maggie Smith

   Article

   Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

   Inserts

   aac3(IV)
   oriT
   attP
   int
   

   Use

   Streptomyces integrating vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXGFPC-5 Pxyl-dcas9 (Spa)

   Plasmid

   #133318

   Purpose

   expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   dcas9 (Streptococcus pasteurianus)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   xylose

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV 4xSTAR-mTFP-NLS-blast

   Plasmid

   #136257

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTFP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-PtH

   Plasmid

   #133391

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2 8xSTAR-mScarletI. PGK-puro

   Plasmid

   #136266

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-BtH

   Plasmid

   #133392

   Purpose

   Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

   Depositor

   Blair Madison

   Article

   Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRPB1-CTD14

   Plasmid

   #112822

   Purpose

   pSMF2 with 14 CTD repeats

   Depositor

   Stephen Fuchs

   Article

   Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   CTD repeats have identical sequence, deleted repe…

   Promoter

   Native promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRPB1-CTD8

    Plasmid

    #112820

    Purpose

    pSMF2 with 8 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRPB1-CTD10

    Plasmid

    #112821

    Purpose

    pSMF2 with 10 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET16b-RPA4/His14 (MSW#949)

    Plasmid

    #208075

    Purpose

    Expression of human Replication Protein A 4 and His-tagged RPA3 in E.coli

    Depositor

    Marc Wold

    Article

    Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

    Insert

    RPA4, RPA14(His-tagged)
    

    Use

    Tags

    10xHis tag on RPA14

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p11d-RPA70/14 (MSW#134)

    Plasmid

    #208076

    Purpose

    Expression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tag

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Insert

    RPA1, RPA3 (RPA1, RPA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY227

    Plasmid

    #184148

    Purpose

    short crRNA generator with spacer LEA2 with WT repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-crRNA-LEA2-WT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Plux2

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLY232

    Plasmid

    #184149

    Purpose

    short tracrRNA generator with WT anti-repeat region

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-WT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. tcTRP24sub8_Cap

    Plasmid

    #175797

    Purpose

    de novo thick circular tandem repeat protein capped to prevent multimerization

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP24sub8_Cap
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2 8xSTAR-sTomato. PGK-puro

    Plasmid

    #136264

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    sTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMX-eSOX2AHK

    Plasmid

    #118826

    Purpose

    Retroviral expression of a mutant of Sox2 that improves efficiency of iPSC generation

    Depositor

    Ralf Jauch

    Article

    Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

    Insert

    eSox2AHK (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    AHK: E46A, I53H

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLhE_AW-pHG165 H118R

    Plasmid

    #90063

    Purpose

    Constitutive expression of a chimeric LacI:CelR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Sousa et al J Mol Biol. 2016 Feb 22;428(4):671-8. do

    Insert

    LLhE_AW/H118R
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I48V/Q55A/Q60R/H118R

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-VEE-GFP

    Plasmid

    #58977

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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