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178,767 results

Showing: 10591 - 10620 of 178767 results

  3. pEF_dCas9

    Plasmid
    #68416
    Purpose
    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68416-plasmid-map-sequence-id-120194

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  6. Beta-2-adrenergic receptor-YFP

    Plasmid
    #67146
    Purpose
    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67146-plasmid-map-sequence-id-121580

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  7. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66080-plasmid-map-sequence-id-436984

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  8. pcDNA3-TORCAR-NLS

    Plasmid
    #64931
    Purpose
    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    Tags
    Cerulean, Nuclear localization signal (NLS), and …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64931-plasmid-map-sequence-id-109697

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  9. pHLSec2-FN-YPet

    Plasmid
    #65421
    Purpose
    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
    Depositor
    Harold Erickson
    Publication
    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    Tags
    YPet
    Expression
    Mammalian
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65421-plasmid-map-sequence-id-115435

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  11. GFP-ATAD2

    Plasmid
    #65370
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATAD2 (ATAD2 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65370-plasmid-map-sequence-id-177053

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  14. pSBtet-GB

    Plasmid
    #60504
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60504-plasmid-map-sequence-id-97543

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  17. pAMPK alpha2 delta312X

    Plasmid
    #60127
    Purpose
    expresses constitutively active AMPKalpha2
    Depositor
    Morris Birnbaum
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPK alpha 2 (Prkaa2 Rat)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Truncated at residue 312
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60127-plasmid-map-sequence-id-317039

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  23. mOrange-pBAD

    Plasmid
    #54751
    Purpose
    Localization: Protein Expression Vector , Excitation: 548, Emission: 562
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and mOrange
    Expression
    Bacterial
    Available since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    54751-plasmid-map-sequence-id-453673

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  27. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  28. mRuby-N1

    Plasmid
    #54581
    Purpose
    Localization: N1 Cloning Vector, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mRuby
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54581-plasmid-map-sequence-id-393318

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Showing: 10591 - 10620 of 178767 results