162,660 results

1. pcDNA3-DN-hCUL4A-FLAG

   Plasmid

   #15821

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin4A (CUL4A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N440

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBE14

   Plasmid

   #174653

   Purpose

   In vivo Cre-lox circularization in E. coli

   Depositor

   Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   Cre
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX4T1-human beta-catenin full length

   Plasmid

   #132609

   Purpose

   expresses human beta-catenin full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

   Insert

   beta-catenin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LV-Sox10MCS5-GFP

   Plasmid

   #115783

   Purpose

   This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Insert

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   

   Use

   Lentiviral

   Tags

   cfos basal promoter conjugated MCS5 promoter fuse…

   Expression

   Mutation

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV3flag8SHARPIN

   Plasmid

   #50014

   Purpose

   Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   SHANK-associated RH domain interactor (SHARPIN Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eGFP-proα1(I)

   Plasmid

   #119843

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM47523

   Plasmid

   #153221

   Purpose

   Level 0 zCas9i vector

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   SpCas9 with introns and 2 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

   Plasmid

   #231128

   Purpose

   Expresses xylanase via a cSAT2.0 scheme

   Depositor

   Xiaofeng Yang

   Article

   Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

   Insert

   xylanase
   

   Use

   Tags

   cSAT2.0

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-G-Rac1

   Plasmid

   #180575

   Purpose

   ddGFP sensor of Rac1

   Depositor

   Won Do Heo

   Article

   Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

   Insert

   ddGFP sensor of Rac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

    Plasmid

    #84469

    Purpose

    pAAV-RAM-EGFP

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1

    Plasmid

    #124650

    Purpose

    Soma-localized C1V1(t/t) for dense expression in excitatory neurons

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Has Service

    AAV9

    Insert

    C1V1
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEVY-U

    Plasmid

    #51230

    Purpose

    bi-directional expression vector for yeast, constitutively active, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC3.1-caggs-Crimson-CAAX

    Plasmid

    #190041

    Purpose

    Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

    Depositor

    Michael Lin

    Article

    Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

    Insert

    Crimson
    

    Use

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 HA-SUMO2-Q90P

    Plasmid

    #36448

    Purpose

    Expresses HA-Sumo2Q90P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutated Q90P

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

18. MSCV-N BBLF1

    Plasmid

    #37923

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BBLF1 (BBLF1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

19. 23 pCOLI_A_N-SUMO_C_STREP

    Plasmid

    #160259

    Purpose

    N-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII, 6xHis, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MBP-TSF-TEVcs-ULK1K46I

    Plasmid

    #171417

    Purpose

    For the purifcation of human ULK1 kinase dead complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    kinase dead

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV:ECFP(FRT)EYFP

    Plasmid

    #31373

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    ECFP(FRT)EYFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV-C 11E6

    Plasmid

    #37873

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 11 E6
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    July 25, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pTOX12

    Plasmid

    #127457

    Purpose

    Encodes Tse2 toxin. Gent-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    tse2 toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p2NIL

    Plasmid

    #20188

    Depositor

    Tanya Parish

    Article

    Parish et al Microbiology (Reading). 2000 Aug;146 ( P

    Type

    Empty backbone

    Use

    Suicide vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-R-Rac1

    Plasmid

    #180576

    Purpose

    ddRFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-PfuX7

    Plasmid

    #182364

    Purpose

    Expresses PfuX7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

    Insert

    PfuX7 DNA polymerase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    V93Q

    Promoter

    T7

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2 xenopus sFRP2

    Plasmid

    #24696

    Depositor

    Edward De Robertis

    Article

    Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

    Insert

    secreted frizzled-related sequence protein 2 (sfrp2 Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only