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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-MjDim1

Plasmid

#189936

Purpose

Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

Depositor

Chuan He

Article

Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

Insert

rsmA (MJ_RS00455 Methanocaldococcus jannaschii)

Tags

6x His tag, FLAG tag, SUMO tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPEP-TEV cNup98 (1-498)

Plasmid

#38037

Depositor

Roderick Lim

Article

Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

Insert

Nucleoporin 98 (NUP98 Human)

Tags

His-tag, 3Cysteine

Expression

Bacterial

Mutation

amino acids 1-498

Promoter

T7

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCerulean-VSVG

Plasmid

#11913

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-mCherry

Plasmid

#220351

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

Plasmid

#218513

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of the Plac_mrfp cassette

Promoter

PgyrA_ermBL-ermB'

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A

Plasmid

#149369

Purpose

Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CBh, PGK, and U6

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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NO lux Sensor

Plasmid

#199790

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Article

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

NO lux Sensor

Use

Synthetic Biology

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-TAPP1-PH

Plasmid

#67662

Purpose

Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-TAPP1-PH

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hM3D(Gq)::RAM-d2tTA

Plasmid

#189627

Purpose

Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

hM3D(Gq)
d2tTA

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

hSyn and pRAM

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Mutation

D10A

Promoter

Pj23119 and Pthl

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1ADA(pCMV5)

Plasmid

#12149

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

Threonine 24 to Alanine (A) and Serine 253 to Asp…

Promoter

CMV

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only
pAM4891 (pCV0003)

Plasmid

#120080

Purpose

Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

Depositor

Susan Golden

Article

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2

Use

Other

Mutation

mobA Y25F

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_Chromo_TAF3_Phd

Plasmid

#179400

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-WT 6KR

Plasmid

#17560

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 6KR (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available Since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only