We narrowed to 11,014 results for: phen
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Plasmid#163719PurposeArabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionineDepositorInsertaraC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
ExpressionBacterialMutationdnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK668
Plasmid#192637PurposeExpresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyMutationSoxS has R93A and S101A mutations and dCas9-NG ha…PromoterBBa_J23107 and Sp.pCas9Available SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBullet-tgn-c
Plasmid#53072Purposedestination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)
Plasmid#160571PurposeVersion of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.DepositorInsertsgRNAscaffold 2.1 scRNA
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
Plasmid#33255DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and phenylalan…PromoterSV40Available SinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectPromotermetallothioneinAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-mRuby3
Plasmid#166139PurposeGateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.DepositorTypeEmpty backboneTagsmRuby3ExpressionYeastAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBullet-end-c
Plasmid#53074Purposedestination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase Non-Cleavable
Plasmid#59826PurposeAllows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
TagsMycExpressionMammalianMutationNon-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2(fs)
Plasmid#127482PurposeNegative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertGal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
ExpressionBacterialMutationLGF2 is frame shifted by the insert of one nucleo…Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(R159E)
Plasmid#172453PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutantDepositorInsertTPD54 (TPD52L2 Human)
TagsmCherry-FKBPExpressionMammalianMutationchanged Arginine 159 to Glutamic AcidPromoterCMVAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV SpCas9
Plasmid#206268PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertSpCas9
UseCRISPR; Recombinant baculovirus productionExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP MPS1 K-R
Plasmid#59819PurposeAllows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.DepositorInsertMps1 (TTK Human)
TagsEmGFPExpressionMammalianMutationKD, catalytically inactive, D664APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mqgDUX4mqg*
Plasmid#21177DepositorInsertDUX4 (DUX4 Human)
ExpressionBacterial and MammalianMutationPoint mutation at nucleotide 6163 (numbering acco…Available SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
human TLNRD1-F250D pET151
Plasmid#159385PurposeExpresses human TLNRD1 F250D mutant in bacterial cellsDepositorInsertTalin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialMutationChanged Phenylalanine 250 to Aspartic Acid (F250D)PromoterT7Available SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TAP(FLAG-HA) CYLD
Plasmid#60031PurposeCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintainedDepositorInsertCylindromatosis (CYLD Human)
UseRetroviralTagsFLAG, HA, and IRES PuroExpressionMammalianPromoterLTR-driven expressionAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD8-dCherry-FRB
Plasmid#100739PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)DepositorInsertCD8A (CD8A Human)
TagsFRB and dCherryExpressionMammalianMutationDark mCherry (K70N) non-fluorescentPromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Left TALE
Plasmid#196496PurposeExpresses left-side TALE-DddAtox Cterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 left TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
ExpressionMammalianAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ND4-FusXTBE-Right TALE
Plasmid#196497PurposeExpresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cellsDepositorInsertCOX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR
ExpressionMammalianAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn2
Plasmid#132712PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)
Plasmid#160577Purpose35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)DepositorInsertPhiC31 PB (attP:TMtb:P35S:attB)
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cg-c
Plasmid#53070Purposedestination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-vac-c
Plasmid#53076Purposedestination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBullet-er-c
Plasmid#53068Purposedestination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(L53P,L67P)
Plasmid#172458PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutantDepositorInsertTPD54 (TPD52L2 Human)
TagsmCherry-FKBPExpressionMammalianMutationchanged Leucines 53 and 67 to proline (breaks coi…PromoterCMVAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pADSL-2XStrep
Plasmid#125684Purposeexpresses human ADSL protein with a C-terminal 2XStrep affinity tagDepositorAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mqg*
Plasmid#21175DepositorInsertDUX4 (DUX4 Human)
ExpressionBacterial and MammalianMutationPoint mutation at nucleotide 6163 (numbering acco…Available SinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*
Plasmid#21172DepositorInsertDUX4 (DUX4 Human)
ExpressionBacterial and MammalianMutationPoint mutation at nucleotide 5114 (numbering acco…Available SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBullet-cyt-c
Plasmid#53066Purposedestination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsECFP and EYFPAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only