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pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

Plasmid

#212962

Purpose

Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_non-targeting spacer

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW101020

Plasmid

#222454

Purpose

Level 1, Position 2 containing 35S::CUP2-p65::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

35S::CUP2-p65::NOS (CUP2 )

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

no

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET-24d-Flag-SKP1

Plasmid

#213690

Purpose

Expresses human SKP1 in bacteria cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1 (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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UbcH5c WT pET28a

Plasmid

#12643

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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MBD2b pcDNA3.1

Plasmid

#78142

Purpose

Mammalian expression of MBD2b

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD2b (MBD2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
HA-Sos2

Plasmid

#32921

Depositor

Dafna Bar-Sagi

Article

Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

Insert

SOS2 (SOS2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLEXY (pDN137)

Plasmid

#72656

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

mCherry-LEXY

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-puro

Plasmid

#17923

Purpose

Insect expression vector

Depositor

David Sabatini

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Drosophila metallothionein gene promoter drives e…

Tags

Expression

Insect

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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HyperNova/pRSETB

Plasmid

#221456

Purpose

Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CamKIIa-jGCaMP8f-WPRE

Plasmid

#176750

Purpose

AAV-mediated expression of GCaMP8f under the CamKIIa promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CamKIIalpha

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 TDP-43 NLS1 YFP

Plasmid

#84912

Purpose

Mammalian expresion of TDP-43 NLS1 YFP

Depositor

Aaron Gitler

Article

Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

Insert

TDP-43 (TARDBP Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

K82A, R83A, K84A

Promoter

CMV

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD2

Plasmid

#197880

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD2 (BRD2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52546

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-HsPeg10rc3

Plasmid

#174859

Purpose

Expresses recoded human Peg10 in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

recoded Homo sapiens PEG10 (PEG10 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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KA1042_pPB-hCMV*1-Esrrb-IV

Plasmid

#124177

Purpose

piggyBac-based Dox-inducible expression of Esrrb

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-IRES-Venus (Esrrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

tetO-CMVmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0

Plasmid

#153285

Purpose

Express the adenosine sensor GRAB_Ado1.0 in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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