165,567 results

1. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

   Plasmid

   #209078

   Purpose

   Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

   Depositor

   Vikram Khurana

   Article

   Lam et al bioRxiv 2022.11.08.515615

   Inserts

   SNCA (SNCA Human)
   NGN2 (Neurog2 Mouse)
   

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   3K mutant: E35K, E46K, E61K

   Promoter

   TRE4G

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLG005 (Retron-TIR)

   Plasmid

   #157883

   Purpose

   Expresses the retron-TIR defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   Retron-TIR
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC18T-mini-Tn7T-Tp-ecfp

   Plasmid

   #65034

   Purpose

   TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   ecfp
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGT-S1

   Plasmid

   #122582

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS_nanorod_105b

   Plasmid

   #120347

   Purpose

   phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

   Insert

   Staple sequences for a DNA origami nanorod
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24b-APEX2

   Plasmid

   #111702

   Purpose

   For IPTG-inducible expression of APEX2 in E. coli

   Depositor

   Jessica Seeliger

   Article

   Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

   Insert

   APEX2
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-EZH2

   Plasmid

   #116738

   Purpose

   Lentiviral expression of EZH2

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSGNluc

   Plasmid

   #186705

   Purpose

   A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

   Depositor

   Maria Leptin, Baubak Bajoghli

   Article

   Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

   Inserts

   GFP
   luciferase
   

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6/V5-DEST-RNF2

   Plasmid

   #31216

   Depositor

   Lynda Chin

   Article

   Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

   Insert

   RNF2 (RNF2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   April 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. GFP-NPM WT

    Plasmid

    #17578

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    EGFP and Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_V5-NEUROD1

    Plasmid

    #215031

    Purpose

    Mammalian expression of human NEUROD1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    neuronal differentiation 1 (NEUROD1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDESTpK7WG2mito

    Plasmid

    #158726

    Purpose

    Plant mitoTALEN vector assembly, Destination vector

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitochondrial presequence in front of attR1 in pk7WG2
    

    Expression

    Plant

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-JEDI-2P-WPRE

    Plasmid

    #179464

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TXNRD1

    Plasmid

    #38863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TXNRD1 (Txnrd1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn-ERT2-iCre-ERT2

    Plasmid

    #178059

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin 1 (Syn) promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-3xFLAG-TurboID-MCS

    Plasmid

    #186813

    Purpose

    CAG promoter-driven expression of TurboID-fused protein (N terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Tags

    3xFLAG-TurboID

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGEN-dPspCas13b-NES-mCherry

    Plasmid

    #233609

    Purpose

    Expression of catalytically-inactive PspCas13b in mammalian cells

    Depositor

    Shintaro Iwasaki

    Article

    Apostolopoulos et al Nat Commun. 2024 Mar 11;15(1):2205. doi:

    Insert

    dPspCas13b-NES-mCherry
    

    Use

    CRISPR

    Tags

    3xFlag and mCherry

    Mutation

    H133A/H1058A

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. -5.5kb-UCP1-GFP

    Plasmid

    #104585

    Purpose

    Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

    Depositor

    Irfan Lodhi

    Article

    Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

    Insert

    UCP1 promoter (Ucp1 Mouse)
    

    Tags

    TurboGFP

    Expression

    Mammalian

    Promoter

    UCP1

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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