We narrowed to 13,271 results for: cDNA

1. hGFAP R239C

   Plasmid

   #169476

   Purpose

   Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   EGFP-hGFAP (GFAP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed R 239 to C

   Promoter

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAPcrRNA_Lmo

   Plasmid

   #126501

   Purpose

   L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

   Plasmid

   #128609

   Purpose

   Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   uORF1-36xMashTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. perbB2K732M-ECFP

   Plasmid

   #40269

   Depositor

   Martin Offterdinger

   Article

   Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

   Insert

   erbB2 (ERBB2 Human)
   

   Use

   Tags

   ecfp

   Expression

   Mammalian

   Mutation

   K732M

   Promoter

   CMV

   Available Since

   Jan. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. POLArIS_NbVim-cp10GFP

   Plasmid

   #171020

   Purpose

   Expresses NbVim-cp10GFP#2 in mammalian cells

   Depositor

   Sumio Terada

   Article

   Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

   Insert

   anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
   

   Use

   Tags

   cp10GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2396-Tier1-PhCMV-4xCOX8_Ypet

   Plasmid

   #169534

   Purpose

   Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized yellow fluorescent protein Ypet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-ECFP-GIT1

   Plasmid

   #15223

   Depositor

   Rick Horwitz

   Article

   Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847

   Insert

   G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
   

   Use

   Tags

   ECFP and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

9. YFP Rab4

   Plasmid

   #99018

   Purpose

   Localization and visualization of trafficking protein Rab4

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Insert

   Rab4 (Rab4 Synthetic, Fly)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

    Plasmid

    #136270

    Purpose

    Expresses ABE and ST2-com modified sgRNA scaffold

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Insert

    ABE and sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ROZA-XL

    Plasmid

    #64194

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ROZA-XL

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. UKNP1.2.1

    Plasmid

    #97356

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLmoCascade-IL1RNcrRNA

    Plasmid

    #126487

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-Cdk9 T186A

    Plasmid

    #28103

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Promoter

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus wGBD

    Plasmid

    #85222

    Purpose

    Venus tagged Cdc-42 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    wGBD (WASL Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eGFP-RAPH1-F1

    Plasmid

    #194860

    Purpose

    Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 1-535

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Use

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDNA3-Cerulean-FHA1-NES

    Plasmid

    #138202

    Purpose

    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    Cerulean-FHA1-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FKBP_cPPVp

    Plasmid

    #118967

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. UKNP1.2.3

    Plasmid

    #97358

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UKNP1.2.4

    Plasmid

    #97359

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. UKNP1.2.5

    Plasmid

    #97360

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3xFKBP-myc

    Plasmid

    #20213

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFKBP (FKBP1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pDEST40-Arl13b89

    Plasmid

    #40874

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Flag-Craf S338A

    Plasmid

    #118335

    Purpose

    Expression of human Craf

    Depositor

    Philip Stork

    Article

    Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

    Insert

    Craf S338A (RAF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S338A

    Promoter

    CMV

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ELC-Fc

    Plasmid

    #8636

    Depositor

    Timothy Springer

    Article

    Manjunath et al J Clin Invest 2001 Sep;108(6):871-8.

    Insert

    CCL19 (CCL19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

30. pAL190

    Plasmid

    #22279

    Purpose

    Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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