We narrowed to 8,677 results for: sgrna

1. pODN-mNG-CfANLN

   Plasmid

   #183837

   Purpose

   Repair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN homology arms with mNeonGreen-linker
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNA_CV2

   Plasmid

   #167165

   Purpose

   pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.

   Depositor

   Alexander Loewer

   Article

   Sheng et al Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 CARD8 PAM

   Plasmid

   #169975

   Purpose

   pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   PAM

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRWJB006

   Plasmid

   #160113

   Purpose

   Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Plant

   Promoter

   35S

   Available Since

   Feb. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK234_038

   Plasmid

   #123917

   Purpose

   Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA-pSV40
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK234_043

   Plasmid

   #123913

   Purpose

   Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA-pSV40
   

   Expression

   Mammalian

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ141-ZmUbi-RZ-Bth

   Plasmid

   #129686

   Purpose

   (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   Empty Backbone
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ141-ZmUbi-RZ-Bh

   Plasmid

   #136378

   Purpose

   Gateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK234_047

   Plasmid

   #124055

   Purpose

   Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   mScarlet sgRNA 2
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTK234_048

    Plasmid

    #124056

    Purpose

    Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 3
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK234_049

    Plasmid

    #124057

    Purpose

    Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mScarlet sgRNA 4
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK234_050

    Plasmid

    #123918

    Purpose

    Encodes the saCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SaCas9-sgRNA-GFPDROPOUT
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK234_051

    Plasmid

    #123919

    Purpose

    Encodes the saCas9 UAS (miniCMV core) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pUAS sgRNA (Sa)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK234_002

    Plasmid

    #123900

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA-GFPDROPOUT
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTK234_010

    Plasmid

    #123903

    Purpose

    Encodes the spCas9 pTRE gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    TetO-sgRNA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MTK234_021

    Plasmid

    #123905

    Purpose

    Encodes the spCas9 pUAS gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA-UAS
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK234_026

    Plasmid

    #123906

    Purpose

    Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    human non targeting sgRNA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK234_040

    Plasmid

    #123915

    Purpose

    Encodes the spCas9 mAzamiGreen gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    mAzamiGreen sgRNA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LRG-2.1T-sgTAF12(human)#4.5

    Plasmid

    #105987

    Purpose

    lentivirally express gRNA targeting human TAF12 HFD with GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting human TAF12-HFD
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN018

    Plasmid

    #91664

    Purpose

    Express sgRNA targeting human SCN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SCN2A (SCN2A Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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