We narrowed to 2,056 results for: ENDOG

1. Halo-TCAB1 HRD

   Plasmid

   #207535

   Purpose

   Homologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC_N_CCDC93

   Plasmid

   #209687

   Purpose

   Expresses MAC-tagged CCDC93 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

   Insert

   CCDC93 (CCDC93 Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeongreen-aGBD(anillin)

   Plasmid

   #129633

   Purpose

   Visualization of active RhoA

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   RhoA binding domain of anillin (712-786)
   

   Expression

   Mammalian

   Promoter

   CMVdel

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-EF1a-HygroR-T2A-TetOn3G

   Plasmid

   #179887

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   Hygromycin resistance

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. doraWT_rescue_construct

   Plasmid

   #190609

   Purpose

   Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Expression

   Insect

   Promoter

   Actin-5c

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3xmNeonGreen-3xrGBD

   Plasmid

   #176101

   Purpose

   Visualization of active Rho

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   rhotekin (Rtkn Mouse)
   

   Expression

   Mammalian

   Promoter

   CMVdel

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJC2-UMPS-3xFLAG

   Plasmid

   #87975

   Purpose

   3rd generation lentiviral vector for Expression of UMPS in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   UMPS (UMPS Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

   Plasmid

   #172066

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGS1222

    Plasmid

    #63791

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Twin-Strep tag followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Twin-Strep tag

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ150-Cas9(dpiRNA)

    Plasmid

    #107940

    Purpose

    eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    Cas9(dpiRNA)
    

    Expression

    Worm

    Promoter

    eft-3

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-Amp_CRISPIE donor B3

    Plasmid

    #172844

    Purpose

    CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mTurquoise2
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-Amp_CRISPIE donor B4

    Plasmid

    #172845

    Purpose

    CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    CRISPIE designer exon (phase 0-0) encoding mNeonGreen
    

    Use

    CRISPR

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDC32B-AtMIR390a-NbSu-2

    Plasmid

    #213400

    Purpose

    Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

    Insert

    Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-PITCh-TUBB4B

    Plasmid

    #207785

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. S12.1_Empty_Bridge

    Plasmid

    #127670

    Purpose

    Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Psicheck2 Sox4 full length 3'UTR

    Plasmid

    #26989

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 Full length 3' UTR (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only