We narrowed to 81,193 results for: TRI

1. pFA6a-link-yECitrine-Hygro

   Plasmid

   #44646

   Depositor

   Kurt Thorn

   Insert

   yECitrine
   

   Expression

   Yeast

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-mPA-GFP-PA-Rac1

   Plasmid

   #22022

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Tags

   6X His and mPA-GFP

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pEF mito-cGFP (matrix)

   Plasmid

   #188904

   Purpose

   Expresses mitochondrial GFP

   Depositor

   Edwin Chapman

   Article

   Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

   Insert

   mitochondrial GFP
   

   Tags

   Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

   Expression

   Mammalian

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-OSF-TRIM5alpha(human)

   Plasmid

   #79034

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human) (TRIM5 Human)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIPZ-bioGEF-SUMO1nc-PURO

   Plasmid

   #208042

   Purpose

   Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   SUMO1nc (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Mutation

   Q94P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTripz Ctrl-IRES-mCherry

   Plasmid

   #187295

   Purpose

   Doxycyclin inducible expression of ctrl IRES-mCherry

   Depositor

   Alpha Yap

   Article

   Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

   Insert

   IRES-mCherry
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CamkII-MacQ-mCitrine

   Plasmid

   #48762

   Purpose

   sensor

   Depositor

   Mark Schnitzer

   Article

   Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

   Insert

   macQ-mCitrine
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   mac-D139Q

   Promoter

   CamKIIa

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. katushka-FMDV-P2A-pTRIP

   Plasmid

   #163522

   Purpose

   Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

   Depositor

   Thomas Weber

   Insert

   stopless Katushka-P2A
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Cas13d-T2A-Citrine

   Plasmid

   #224565

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   Cas13d-T2A-Citrine
   

   Use

   CRISPR

   Tags

   T2A-Citrine

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-FLAG-Sestrin2-L261A

    Plasmid

    #73671

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L261A changed Leucine 261 to Alanine

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. C1-Lyn-mCitrine-EEVEE-LifeAct

    Plasmid

    #155226

    Purpose

    Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LMPd Ametrine Got2 shRNA#3

    Plasmid

    #220593

    Purpose

    Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

    Depositor

    Russell Jones

    Article

    Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

    Insert

    Got2 shRNA (Got2 Mouse)
    

    Use

    Retroviral

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-Beta4-spectrin [N393/2R]

    Plasmid

    #177541

    Purpose

    Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/2.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid

    #22017

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-NTOM20-mVenus-Zdk3

    Plasmid

    #81012

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk3
    

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. SspB-mCherry-DHPH1-TRIO

    Plasmid

    #176111

    Purpose

    Heterodimerization with iLID, Rac1 and RhoG activation

    Depositor

    Dorus Gadella

    Insert

    TRIO (TRIO Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-FKBP-TRIF(Nterm)

    Plasmid

    #221244

    Purpose

    Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TRIF N-terminus (1-222) (Ticam1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-FLAG-Sestrin2-E451A

    Plasmid

    #73673

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only