We narrowed to 13,937 results for: cas9

1. pX165-xCas9

   Plasmid

   #140568

   Purpose

   Expresses xCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Mutation

   A262T R324L S409I E480K E543D M694I E1219V

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSAY-vasaCas9sv40

   Plasmid

   #173669

   Purpose

   Cas9 expression vector for Anopheles

   Depositor

   Eric Marois

   Insert

   Cas9 under control of vasa promoter and SV40 terminator
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   vasa

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9m2-VP64

   Plasmid

   #47317

   Purpose

   Cas9m2 Activator

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m2-VP64
   

   Use

   CRISPR

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pEC-K-CBP_CjeCas9

   Plasmid

   #83043

   Purpose

   Expresses Campylobacter jejuni Cas9 (Cje Cas9).

   Depositor

   Jennifer Doudna

   Article

   Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

   Insert

   CjeCas9
   

   Expression

   Bacterial

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

5. p426_Cas9_gRNA-HIS3b

   Plasmid

   #87402

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p426_Cas9_gRNA-ARS1309a

   Plasmid

   #87399

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1309a sequence CCTGTGGTGACTACGTATCC in yeast chromosome 13.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nsp2-SmuCas9_AAV

   Plasmid

   #192144

   Purpose

   Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nsp2-SmuCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-6-AtSpCas9-9

   Plasmid

   #197778

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   AtSpCas9
   

   Expression

   Plant

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p426_Cas9_gRNA-ARS1622b

    Plasmid

    #87403

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAct:dCas9-scFv

    Plasmid

    #78900

    Purpose

    Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scFV-VP64
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMPRSS2 g4 + Cas9

    Plasmid

    #153018

    Purpose

    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + TMPRSS2 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. peSpCas9-J23109

    Plasmid

    #113152

    Purpose

    Plasmid constitutively expressing eSpCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpCas9
    

    Expression

    Bacterial

    Mutation

    K810A & K1003A & R1060A

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_dCas9-2xAM_hIRF-1

    Plasmid

    #92221

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9 dFE

    Plasmid

    #190137

    Purpose

    Expresses SpCas9-T4pol (dFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-T4pol (dFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p426_Cas9_gRNA-ARS911b

    Plasmid

    #87394

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTaCas9-D10A_5

    Plasmid

    #91170

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas9m3-VP64

    Plasmid

    #47318

    Purpose

    Cas9m3 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m3-VP64
    

    Use

    CRISPR

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid

    #155049

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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