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TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA

Plasmid

#158390

Purpose

Inducible Bxb1 integrase for mammalian cells

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

Bxb1-ecDHFR

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

Plasmid

#205152

Purpose

Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

FRT-GFP-FRT-uORF-dCBEevoCDA1

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-crRNA

Plasmid

#194967

Purpose

The plasmid expresses Cas12j-8-crRNA expression elements.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB-LpxE

Plasmid

#128389

Purpose

Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Inserts

PglB oligosaccharylktransferase
LpxE phosphatase

Tags

Flag tag

Expression

Bacterial

Promoter

Ptac and pBAD

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_BirA

Plasmid

#136537

Purpose

Lentiviral expression vector for an inducible BirA

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pKEK3230

Plasmid

#234102

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

FuGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLCRISPR-CMV

Plasmid

#102609

Purpose

Control lentivector expressing Cas9 and gRNA scaffold

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP8923

Plasmid

#220904

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRG713_lexO-HO_LexA-TF_URA3MX

Plasmid

#154821

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2

Plasmid

#191338

Purpose

Expresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice cultures

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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R26-pCAG-Ceru-10unit-BGHpA

Plasmid

#158387

Purpose

10-unit intMEMOIR recording array for mammalian cells

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

intMEMOIR 10-unit array

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pET-CV-SNAP25

Plasmid

#168031

Purpose

Expresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-SNAP25(A141-G206)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUB_smFLAG_ActB_MS2

Plasmid

#81083

Purpose

Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_ActB_24xMS2

Expression

Mammalian

Available Since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-I-N-CAAX

Plasmid

#41686

Purpose

Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145N-IntersectinDH-Dronpa145N-CAAX

Tags

CAAX box

Expression

Mammalian

Mutation

K145N on Dronpa

Promoter

CMV

Available Since

Dec. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV intron renilla TRICK CTE polyA

Plasmid

#84443

Purpose

TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

Renill-TRICK

Use

Luciferase; Frt

Expression

Mammalian

Promoter

Tet-CMV

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Promoter

lac

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLG018 (AVAST type 2)

Plasmid

#157896

Purpose

Expresses the AVAST type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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