We narrowed to 10,723 results for: SEC

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416L

Plasmid

#81036

Purpose

mammalian expression of LOV V416L, a mutant with super long relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416L

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416L

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD3-dU6:3gRNA

Plasmid

#49410

Purpose

expression of gRNA under control of the Drosophila U6:3 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-Cyc

Plasmid

#33156

Depositor

Michael Rosbash

Article

Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

Insert

Cyc (cyc Fly)

Use

Retroviral

Tags

6xHIS and V5

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-D1cpV

Plasmid

#37479

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1cpV second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Article

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Expression

Bacterial

Promoter

sppA

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVie-NcanGFP

Plasmid

#235983

Purpose

Expresses the hyaluronan-binding domain of Neurocan fused to eGFP under the CMV promoter. It contains a sequence for secretory pathway, so the probe is exported to the extracellular space

Depositor

Federico Soria

Article

Fernández-Ballester et al bioRxiv 2025.01.31.635882; doi: 10.1101/

Insert

Neurocan-GFP

Use

AAV

Tags

GFP

Promoter

CMVie

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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SCORE

Plasmid

#40232

Depositor

Wolfhard Almers

Article

An et al Science. 2004 Nov 5;306(5698):1042-6.

Insert

Snap25b (SNAP25 Human)

Tags

CFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP2

Plasmid

#59037

Purpose

for baculovirus expression of CAMSAP2 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

p65AD-Zip (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-GFP

Plasmid

#130277

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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