165,343 results

1. pAAV.TBG.PI.Null.bGH

   Plasmid

   #105536

   Purpose

   empty AAV vector with TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   None
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TBG

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET47b-T7-enAsCas12f

   Plasmid

   #204642

   Purpose

   Expression of enAsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-CMV mCherry-GFP-LC3B WT

   Plasmid

   #123230

   Purpose

   Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LARS_pNIC-Bio3

   Plasmid

   #153052

   Purpose

   Expression construct for producing Avi tagged Leucyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   LARS (LARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Not full length protein

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SF3B3-1

   Plasmid

   #156004

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SF3B3 (SF3B3 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6.3 GCN1-3xFlag_IRES-iRFP

   Plasmid

   #198388

   Purpose

   GCN1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   GCN1 (GCN1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Xl-VSP1

   Plasmid

   #51880

   Purpose

   expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. myc-Rictor corrected

    Plasmid

    #11367

    Purpose

    Mammalian expression of myc tagged human RICTOR

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPtPBR2

    Plasmid

    #80387

    Purpose

    Episome vector for Phaeodactylum tricornutum (pBR322-based)

    Depositor

    Philip Weyman

    Type

    Empty backbone

    Use

    Synthetic Biology; ; episomal vector for phaeodac…

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_CBR1_WT_V5

    Plasmid

    #82945

    Purpose

    Gateway Donor vector containing CBR1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CBR1 (CBR1 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCx55.5-TrpR

    Plasmid

    #86373

    Purpose

    for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    TrpR
    

    Use

    Zebrafish

    Promoter

    cx55.5

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-C1-p63RhoGEF

    Plasmid

    #67896

    Purpose

    Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

    Insert

    mCherry-p63RhoGEF
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUX19

    Plasmid

    #36328

    Depositor

    Gary Roberts

    Type

    Empty backbone

    Use

    Suicide vector for r. rubrum

    Expression

    Bacterial

    Promoter

    reduce strength lacZ

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

16. 1572 pSG5L Flag HA GGA2

    Plasmid

    #11184

    Depositor

    William Sellers

    Insert

    GGA2 (GGA2 Human)
    

    Tags

    HA and flag

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_2pabPAC

    Plasmid

    #158969

    Purpose

    2P-activatable bPAC for AAV

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPAC
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TARS_pNIC-Bio3

    Plasmid

    #153057

    Purpose

    Expression construct for producing Avi tagged Threonyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    TARS (TARS1 Human)
    

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-LSS-SGFP2

    Plasmid

    #112943

    Purpose

    Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Insert

    LSS-GFP2 mCherry fusion
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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