We narrowed to 13,211 results for: CAR

1. pACB109

   Plasmid

   #222208

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (K10N)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Lys10 to Asn

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACB110

   Plasmid

   #222209

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (G258D)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Gly258 to Aspartate

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACB111

   Plasmid

   #222210

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(G76V)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed glycine 76 to valine

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACB112

   Plasmid

   #222211

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(S11R)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Serine 11 to Arginine

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACB113

   Plasmid

   #222212

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(W15C)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Tryptophan 15 to Cysteine

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJM166B

   Plasmid

   #218123

   Purpose

   For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   FRB (MTOR Human)
   

   Tags

   rpoZ

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-SREBP-2-GFP (A1134-1135)

   Plasmid

   #204203

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Tags

   T7x3 and eGFP

   Expression

   Mammalian

   Mutation

   Gly to Ala 1134, 1135

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halotag-6xHp

   Plasmid

   #214414

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to Halotag

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   6x Spastin aa 43-92 (SPAST Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mApple-1xHp

   Plasmid

   #214409

   Purpose

   Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   Spastin aa 43-92 (SPAST Human)
   

   Tags

   mApple

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN

    Plasmid

    #206967

    Purpose

    Expresses TadA7.10 and SpG cas9N by the constitutive CMV promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    CMV, TadA7.10, SpG N
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-TadA8e-SpG N aa2-713-InteinN

    Plasmid

    #206968

    Purpose

    Expresses TadA8e and SpG cas9N by the constitutive CMV promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    CMV, TadA8e, SpG N
    

    Use

    AAV

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1

    Plasmid

    #206974

    Purpose

    Expresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

    Depositor

    Yuxuan Guo

    Article

    Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

    Insert

    NG aa714-1368, U6, Lmna sgRNA1
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-Creb5(HA)

    Plasmid

    #195022

    Purpose

    Gateway vector containing HA-tagged Creb5

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GL4.10 E2E1- Prg4 promoter-luciferase

    Plasmid

    #195035

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.10 E3E2E1-Prg4 promoter-luciferase

    Plasmid

    #195036

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

    Plasmid

    #195037

    Purpose

    Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
    

    Use

    Luciferase

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV.5’eGFP/sgLMNA

    Plasmid

    #170546

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of LMNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV.5’eGFP/sgVIM

    Plasmid

    #170548

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of VIM
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET30b jGCaMP8f L27A

    Plasmid

    #204138

    Purpose

    Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Tags

    N-terminal His tag

    Expression

    Bacterial

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    T7

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CMV-jGCaMP8f L27A

    Plasmid

    #204140

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    CMV

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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