165,343 results

1. LAMP-mCherry-CRY2

   Plasmid

   #102249

   Purpose

   Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTet-SNAP-Cep72

   Plasmid

   #136819

   Purpose

   Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep72 (CEP72 Synthetic, Human)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB337

   Plasmid

   #68061

   Purpose

   Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter

   Depositor

   Takayuki Kohchi

   Article

   Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ379

   Plasmid

   #74215

   Purpose

   Combined sgRNA/Cas9 vector with NatMX marker

   Depositor

   Mikel Zaratiegui

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSOX11-donor

   Plasmid

   #176360

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   SOX11 (SOX11 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. gCH128 (non-targeting crRNA)

   Plasmid

   #217339

   Purpose

   Non-targeting crRNA

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   crNT-3
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-TRE-DIO-Cas9

   Plasmid

   #166601

   Purpose

   Encodes Cre-dependent Cas9 under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   Cas9
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-Brainbow-1.1 M

   Plasmid

   #18722

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Expression

    Mammalian

    Mutation

    * see comments

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab4B

    Plasmid

    #61801

    Purpose

    Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab4B (RAB4B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACUH-GFP1-10 mitochondrial matrix

    Plasmid

    #172063

    Purpose

    UAS vector to express GFP1-10 in the mitochondrial matrix

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP1-10 mitochondrial matrix
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCDH-puro-Bcl-XL

    Plasmid

    #46972

    Purpose

    lentiviral expression of Bcl-XL

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. BM00654

    Plasmid

    #155339

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ1661-sgMUC4-E3(F+E)

    Plasmid

    #51025

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

    Depositor

    Stanley Qi, Bo Huang

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    optimized sgRNA (MUC4 Human, Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MED12_pLX307

    Plasmid

    #98350

    Purpose

    Lentiviral expression of MED12

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MED12 (MED12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R1392Q

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-2xFLAG-2xSTREP-CCND2

    Plasmid

    #172629

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRG/III-hs-MBP-CB2-HF

    Plasmid

    #121682

    Purpose

    Bacterial expression of cannabinoid receptor CB2 fusion protein

    Depositor

    Reinhard Grisshammer, Richard Henderson

    Article

    Calandra et al Biotechnology Letters

    Insert

    peripheral cannabinoid receptor (CNR2 Human)
    

    Tags

    His6 Flag tag

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV-miR-142

    Plasmid

    #117056

    Purpose

    Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

    Depositor

    Michele De Palma

    Article

    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

    Insert

    miR-142-3p - OFP (Mir142 Mouse)
    

    Use

    Lentiviral

    Promoter

    SFFV promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pelB-MBP-HtrA-ss

    Plasmid

    #137066

    Purpose

    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature HtrA: lacks the HtrA signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. WT-MEK1-V5

    Plasmid

    #217297

    Purpose

    Lentivirus vector for expression of MEK1

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

    Plasmid

    #172985

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pACEBac1-GCP2

    Plasmid

    #178068

    Purpose

    Insect cell expression of GCP2

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    GCP2 (TUBGCP2 Human)
    

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCDNA4/Full length FVIII

    Plasmid

    #41036

    Depositor

    Robert Peters

    Article

    Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

    Insert

    FVIII (F8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMRE-Tn5-163

    Plasmid

    #118542

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCW57.1 _Gata6

    Plasmid

    #73537

    Purpose

    Inducible lentiviral expression of Gata6

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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