162,682 results

1. pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

   Plasmid

   #216666

   Purpose

   Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   MyoD (Myod1 Mouse)
   miRFP703
   PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AiP14948: pAAV-AiE0441h_3xC2-minBG-DIO-SYFP2-WPRE3-BGHpA (Alias: CN4948)

   Plasmid

   #214867

   Purpose

   Expression of SYFP2 in striatal medium spiny neurons in a Cre dependent manner; useful for intersectional labeling

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AiP15138: pAAV-AiE0873m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5138)

   Plasmid

   #214868

   Purpose

   Expression of iCre(R297T) in striatal cholinergic neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R297T

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BS-TRP1-TetOff(7repeats)-GFP-Pho8

   Plasmid

   #207011

   Purpose

   Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDB179-CaPETaseM9

   Plasmid

   #218707

   Purpose

   Encodes codon optimized CaPETaseM9 enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   CaPETaseM9
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCBE3-NG

   Plasmid

   #218159

   Purpose

   This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / L1111…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GPR143-DuET

   Plasmid

   #213264

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR143 (GPR143 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

   Plasmid

   #216477

   Purpose

   lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNLF1-N_CTNNB1:1-781

   Plasmid

   #211024

   Purpose

   Mammalian expression of full-length human CTNNB1. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   CTNNB1:M1-L781 (CTNNB1 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GABA(A)R, Alpha1 scFv [N95/35]

    Plasmid

    #199430

    Purpose

    Mammalian expression of GABA(A)R, Alpha1 scFv. Derived from hybridoma N95/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGABA(A)R, Alpha1 (Human) (GABRA1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Neuroligin-2 scFv [L107/95]

    Plasmid

    #206764

    Purpose

    Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/95 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMuLE (L1-L4) 4X PRE

    Plasmid

    #206167

    Purpose

    pMuLE L1-L4 entry plasmid containing 4 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE
    

    Use

    Gateway cloning entry clone

    Tags

    Expression

    Mutation

    Promoter

    2YBTATA

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Kv4.2 K+ channel (external) scFv [K57/41]

    Plasmid

    #206771

    Purpose

    Mammalian Expression of Kv4.2 K+ channel (external) scFV. Derived from hybridoma K57/41 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Kv4.2 K+ channel (external) (Homo sapiens) recombinant scFV (KCND2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Neurexin-1-Beta scFv [N170A/26]

    Plasmid

    #199425

    Purpose

    Mammalian expression of Neurexin-1-Beta scFv. Derived from hybridoma N170A/26.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingNeurexin-1-Beta (Human) (NRXN1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD649-HAsp-SEMA3A-COMP5AP-AviTag-9xHis

    Plasmid

    #157536

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3A (SEMA3A Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GABA(A)R, Gamma2L/S scFv [N452/73]

    Plasmid

    #190555

    Purpose

    Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458_ZNF644_2

    Plasmid

    #86306

    Purpose

    Encodes gRNA for 3' target of human ZNF644

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ZNF644 (ZNF644 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PX458_ZNF644_1

    Plasmid

    #86305

    Purpose

    Encodes gRNA for 3' target of human ZNF644

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against ZNF644 (ZNF644 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHEH-4

    Plasmid

    #36283

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Insert

    HEH-4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHIV-dTomato

    Plasmid

    #21374

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMX-Baf155

    Plasmid

    #25856

    Depositor

    Hans Schöler

    Article

    Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

    Insert

    Brg1 associated factor 155 (Smarcc1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-REPORT(PGK/CMV)-12xHRE

    Plasmid

    #172335

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ACBE-Q

    Plasmid

    #204608

    Purpose

    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid

    #141391

    Purpose

    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-N (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAIP hIL4 co

    Plasmid

    #74169

    Purpose

    Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL4 (IL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-P-RAN-GFP1

    Plasmid

    #106408

    Purpose

    P-RAN-GFP1 (RANbody GFP1::vHRP)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    P-RAN-GFP1
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge