We narrowed to 12,778 results for: NUC
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Plasmid#236764PurposeA piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.DepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromotermouse U6 promoter, human U6 promoter, CAG promoterAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only
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pCAGEN_NES_V5_GFP
Plasmid#235632PurposeEncodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.DepositorInsertGFP
TagsNES-V5ExpressionMammalianAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag-TUBG2
Plasmid#229855PurposeTUBG2DepositorInsertTUBG2 (TUBG2 Human)
TagsFlagExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDW108
Plasmid#227194PurposeTemplate for multi-nucleosome arrayDepositorInsert64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
ExpressionBacterialPromoterPlacAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Sga-Cas9
Plasmid#227005PurposeMammalian expression of Nuclease active S. gallolyticus Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Sin-Cas9
Plasmid#227006PurposeMammalian expression of Nuclease active S. iniae Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Spa-Cas9
Plasmid#227007PurposeMammalian expression of Nuclease active S. parasanguinis Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Sub-Cas9
Plasmid#227008PurposeMammalian expression of Nuclease active S. uberis Cas9DepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLSExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:ChrH
Plasmid#225084PurposeExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coliDepositorInsertChrH
TagsHis6-ThrombinExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-nCas9n
Plasmid#217487PurposeExpression of nuclear nCas9nDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-hHNF4aORF-1
Plasmid#206959PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.DepositorInsertHNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
ExpressionBacterialPromoterT7Available SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-MBP-rATOM(WDR5)
Plasmid#209710PurposeMammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signalDepositorInsertNLS-MBP-rATOM(WDR5)
TagsNLS-MBPExpressionMammalianAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.13_GagZip-MCP_Zip-P2A-eGFP
Plasmid#205528PurposeExpress HIV GagZip-MCP_Zip-P2A-eGFPDepositorInsertGagZip-MCP_Zip
ExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP
Plasmid#205529PurposeExpress HIV GagZip-dp1dp6-MCP-P2A-eGFPDepositorInsertGagZip-dp1dp6-MCP
ExpressionMammalianMutationNucleocapsid replaced with leucine zipper, p1 and…PromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE1177
Plasmid#202775PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRExpressionPlantAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2911
Plasmid#161764PurposeMODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.DepositorInsertrolD:TREX2
ExpressionBacterialPromoterrolDAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1a/hU6Available SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_MCS_version1
Plasmid#197937PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.DepositorInsertpcoCasphi-2
UseCRISPRExpressionPlantPromoterpUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC2
Plasmid#191857PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC6
Plasmid#191861PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC5
Plasmid#191860PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC4
Plasmid#191859PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC1
Plasmid#191856PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pElectra1-LaminA
Plasmid#179482PurposeNuclear envelope labeling with blue fluorescent protein Electra1DepositorInsertElectra1-LaminA
TagsElectra1ExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB106
Plasmid#185065PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domainDepositorInsertNUP1
TagsGSTExpressionBacterialMutationNup1 C-terminal region, truncated so C-terminal h…Available SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1
Plasmid#183404PurposeDual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PAG-E2-Crimson-3XNLS-pA
Plasmid#183801PurposeAAV vector to express nuclear localized E2-Crimson from PAG promoterDepositorInsertE2-Crimson-NLS
UseAAVTagsNLSAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-pntAB
Plasmid#158609PurposeLow phosphate induction of pntABDepositorInsertpntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
ExpressionBacterialPromoterInsulated ugpBp from E. coliAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only