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We narrowed to 18,759 results for: nls

Showing: 1441 - 1480 of 18759 results
  1. pRD397

    Plasmid
    #163104
    Purpose
    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mTurquoise2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Promoter
    CAG and EF1a
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-ICUE3-NLS
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP B
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. RANLS

    Plasmid
    #50843
    Purpose
    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  7. 864 pSG5L HA NLS PTEN

    Plasmid
    #10933
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTEN (PTEN Human)
    Tags
    HA and NLS
    Expression
    Mammalian
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  8. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. MTK3_008

    Plasmid
    #123723
    Purpose
    Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-VP16-EL222
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Expression
    Plant
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pAAVss-G1B3-nlsCre-WPRE-miR124T-SYNpolyA

    Plasmid
    #245080
    Purpose
    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Insert
    nls-Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. SIN-cPPT-G1B3-nlsCre-WPRE-miR124T

    Plasmid
    #245072
    Purpose
    CRE recombinase with miRNA-124 target sequence to repress transgene expression in neurons
    Depositor
    Insert
    nls-CRE
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Human G1B3 promoter
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pCSnERT(#186)

    Plasmid
    #184057
    Purpose
    to express cyclofen-inducible fusions in mammalian cells or fish eggs
    Depositor
    Insert
    nls-ERT2
    Use
    Synthetic Biology
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pGGD_RT_2NLS

    Plasmid
    #136977
    Purpose
    Goldengate/Greengate module nuclear localization signal
    Depositor
    Insert
    SV40 NLS
    Expression
    Bacterial
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pcDNA3-Akt-STOPS-NLS

    Plasmid
    #175007
    Purpose
    Nuclear-targeted Akt-STOPS.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Akt-STOPS-NLS
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pSBbi-FoxO1_1R_13A_3D

    Plasmid
    #106279
    Purpose
    Fluorescent fusion protein for FoxO1
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Foxo1a (Foxo1 Mouse)
    NLS-mCherry-NLS-P2A-Puromycin
    Tags
    Clover fluorescent protein and NLS
    Expression
    Mammalian
    Mutation
    Deleted amino acids 401-636, T24A, S209A, H212R, …
    Promoter
    EF1a/RPBSA
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1441 - 1480 of 18759 results