We narrowed to 28,411 results for: REP

1. pcDNA3.1-2xFLAG-2xSTREP-CDK2

   Plasmid

   #172616

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. betaTrCP mVenus mutant reporter

   Plasmid

   #192459

   Purpose

   Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

   Depositor

   Steven Cappell

   Article

   Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

   Insert

   CdC25B
   

   Use

   Lentiviral

   Tags

   SV40 NLS and mVenus

   Expression

   Mammalian

   Mutation

   aa198-338 of CDC25B. betaTrCP degron sequence (aa…

   Promoter

   EF1alpha

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SOX2 TALE Repressor (KRAB 11-75)

   Plasmid

   #42946

   Depositor

   Feng Zhang

   Article

   Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

   Insert

   SOX2 TALE Repressor (KRAB 11-75)
   

   Use

   Lentiviral

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN ReP5-GFP-GluR1

   Plasmid

   #24004

   Depositor

   Robert Malinow

   Article

   Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

   Insert

   GluR1 (Gria1 Rat)
   

   Use

   Sindbis virus production

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

5. 6xHis(TEV)-dDrp1-StrepII

   Plasmid

   #174421

   Purpose

   Expresses D. melanogaster Drp1 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1 (Drp1 Fly)
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC18T-mini-Tn7T-Gm-REP

   Plasmid

   #64966

   Purpose

   mini-Tn7 vector with oriR6K, for attTn7 site determination

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   oriR6K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pMS17: pTwinStrep-SUMO-AvTnsD

   Plasmid

   #168150

   Purpose

   AvTnsD expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsD
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMS15: pTwinStrep-SUMO-AvTnsB

   Plasmid

   #168148

   Purpose

   AvTnsB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMS16: pTwinStrep-SUMO-AvTnsC

   Plasmid

   #168149

   Purpose

   AvTnsC expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   AvCAST TnsC
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMS14: pTwinStrep-SUMO-AvTnsA

    Plasmid

    #168147

    Purpose

    AvTnsA expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsA
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP Y93H GC editing reporter

    Plasmid

    #198884

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Y93H, Gly92(GGT-to-GGG)

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX385 Streptococcus pasteurianus Cas9

    Plasmid

    #68322

    Purpose

    Streptococcus pasteurianus Cas9

    Depositor

    Feng Zhang

    Insert

    SpaCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOET3-N-TEV-Twin-Strep

    Plasmid

    #203323

    Purpose

    Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMS32: pTwinStrep-SUMO-PmcTnsC

    Plasmid

    #168165

    Purpose

    PmcTnsC expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsC
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lepr peak reporter-eGFP

    Plasmid

    #196584

    Purpose

    Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    eGFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDFNKI-his-3C-LIC-strep

    Plasmid

    #108705

    Purpose

    Expression of a His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-2xSTREP-CDK4

    Plasmid

    #172615

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS31: pTwinStrep-SUMO-PmcTnsAB

    Plasmid

    #168164

    Purpose

    PmcTnsAB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsAB
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC8-TwinStrep-3C-DrHDAC6 (40-831)

    Plasmid

    #180226

    Purpose

    Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

    Depositor

    Patrick Matthias

    Article

    Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

    Insert

    Histone deacetylase 6 (hdac6 Zebrafish)
    

    Use

    Tags

    TwinStrep-3C

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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