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We narrowed to 2,422 results for: dcas9

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Showing: 721 - 740 of 2422 results

  1. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

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  2. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

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  4. pdCas9-GFP-VPR

    Plasmid
    #181907
    Purpose
    Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
    Depositor
    Karsten Rippe
    Publication
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181907-plasmid-map-sequence-id-359690

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  10. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73223-plasmid-map-sequence-id-135055

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  11. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Promoter
    xylose
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133317-plasmid-map-sequence-id-266835

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  12. pPB-CAG-dCas9-VPR-wpA-pPuroTK

    Plasmid
    #214127
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214127-plasmid-map-sequence-id-424240

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  15. VPR_dCas9

    Plasmid
    #134601
    Purpose
    Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker
    Depositor
    Lasse Ebdrup Pedersen
    Publication
    Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VPR-dCas9-2A-ZsGreen-DR
    Tags
    VPR and ZsGreen1-DR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134601-plasmid-map-sequence-id-269007

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  19. pAPH021

    Plasmid
    #234346
    Purpose
    Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK
    Depositor
    Jerome Fox
    Publication
    Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12
    Inserts
    Type II dCas9 sgRNA
    Type II dCas9 sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lacUV5
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234346-plasmid-map-sequence-id-470058

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Showing: 721 - 740 of 2422 results