We narrowed to 10,409 results for: SEC

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CD44I-c014

Plasmid

#175451

Purpose

Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220. Can be biotinylated.

Depositor

Opher Gileadi

Insert

CD44 (CD44 Human)

Tags

AviTag-His6

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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CD44U-c011

Plasmid

#175454

Purpose

Protein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.

Depositor

Opher Gileadi

Insert

CD44 (CD44 Human)

Tags

AviTag-His6

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0764

Plasmid

#177030

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase; CYP72C

Use

Synthetic Biology

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E2E3E4 PUb-DsRed

Plasmid

#173665

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
DsRed

Expression

Insect

Mutation

Nix isoforms with 4 exons

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGal4BD 1-147

Plasmid

#165819

Purpose

1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Gal4BD 1-147

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4AD 768-881

Plasmid

#165820

Purpose

768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL4AD 768-881

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Promoterless TP10

Plasmid

#165787

Purpose

Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless T10

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-Gal80

Plasmid

#169030

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pre_II_CS3_7746_v3

Plasmid

#126856

Purpose

7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffolds

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

II_CS3_7746_v3

Use

Synthetic Biology

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

Plasmid

#132426

Purpose

Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

Ubiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Myo 9 Avi+Flag

Plasmid

#134969

Purpose

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

Depositor

Martin Bähler

Article

Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

Insert

Myo 9 (hum-7 Nematode)

Tags

Avi and Flag

Expression

Insect

Promoter

Polyhedrin

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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U6d-T

Plasmid

#117212

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

u6d promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N64_trigger

Plasmid

#132746

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 trigger RNA

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_trigger

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N19_switch

Plasmid

#132744

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 19 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N19_switch

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_ToeRepG2_N64_switch

Plasmid

#132743

Purpose

T7 RNAP-driven expression of second-generation toehold repressor index 64 switch RNA with a GFP-ASV reporter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

ToeRepG2_N64_switch

Expression

Bacterial

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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LC8-like

Plasmid

#133115

Purpose

Human LC8-like protein, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

LC8-like (LOC392067 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 21-55 eGFP SV40

Plasmid

#132653

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA UTR delta 41-55 eGFP SV40

Plasmid

#132652

Purpose

Expresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTR

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

eGFP

Expression

Insect

Promoter

MtnA

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS attB V5 dDispatched WT HA

Plasmid

#126411

Purpose

Expression of wild type dDispatched HA in Drosophila.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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