We narrowed to 8,464 results for: gnal
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Plasmid#18959DepositorInsertBrevican C-terminus (Bcan Rat)
ExpressionMammalianMutationRat brevican C-terminus: Signal peptide aa 1-22 …Available SinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-RLuc8N-NES
Plasmid#138212PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertPKAsub(T/A)-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TA1-DuET
Plasmid#213382PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO2-Flp
Plasmid#149296PurposeCre-dependent expression of Flp recombinase.DepositorInsertFlp recombinase (flp Budding Yeast)
UseAAVMutationDerived from the mouse codon-optomized FlpO. The…Available SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-CIAO-Flp
Plasmid#149294PurposeCre-dependent expression of Flp recombinase.DepositorInsertFlp recombinase (flp Budding Yeast)
UseAAVMutationDerived from the mouse codon-optomized FlpO. The…Available SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW581
Plasmid#198818PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kineticsDepositorInsert15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
ExpressionWormAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-STAT3-3xFlag
Plasmid#99260PurposeAdenoviral vector encoding Flag-tagged murine STAT3DepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAdenoviralTags3x-FlagPromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1a
Plasmid#61562PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
TagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-LAR-GECO1.2
Plasmid#61245PurposeExpresses LAR-GECO1.2 in the mitochondria in mammalian cellsDepositorInsertLAR-GECO1.2
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to R-GECO1.2: N45I/A47R/E1…PromoterCMVAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HRE-dUnaG
Plasmid#124372PurposeUnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signalingDepositorInsertHRE-dUnaG
UseLentiviralTagsPEST-degron and Myc tagPromoterHRE (HIF responsive element 5x + CMV minimal prom…Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Lamp2b-HA
Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
TagsFlag, HA, and Lamp2 signal peptideExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRCaMP1b
Plasmid#63136PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRCaMP1b
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-Egfr(Velvet)
Plasmid#18788DepositorInsertepidermal growth factor receptor (Egfr Mouse)
TagsFLAGExpressionMammalianMutationD833G; signal sequence removed (aa1-24)Available SinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIhsp+LuxG13
Plasmid#49999PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertBacterial luciferase operon + G13 promoter
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterG13 from M. marinum G13 promoter driving luxCAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only