We narrowed to 10,841 results for: esp
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Plasmid#173787PurposeMammalian expression of SARS-CoV-2 Spike protein South African variant version 3DepositorInsertSpike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
Plasmid#145843PurposeLentiviral vector genome to ectopically express human TMPRSS2 variant 1DepositorInserthuman TMPRSS2 variant 1 (TMPRSS2 Human)
UseLentiviralExpressionMammalianMutationG8VPromoterSFFVAvailable SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-Q61L-shortlinker
Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.Available SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-ER-Rac1-P29S-shortlinker
Plasmid#128583PurposeFor mammalian cell expression of P29S mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Proline29 to Serine in the RAC1 moiety.Available SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
132_pAAV-ProB2-CatCh-GFP-WPRE
Plasmid#125922PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVPromoterProB2Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1 Lck-GCaMP5G
Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorInsertLck-GCaMP5G
ExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable SinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpRY-His
Plasmid#179320PurposePlasmid for bacterial expression and purification of SpRY-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpRY
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…Available SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
123_pAAV-ProC1-CatCh-GFP-WPRE
Plasmid#125937PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVPromoterProC1Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 RBD (bio-His)
Plasmid#166857PurposeHuman expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.DepositorInsertSARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
Tags6xHis and Biotinylation siteExpressionMammalianPromoterCMVAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
TagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…Available SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-FLEX-mCh-T2A-FLAG-hMOR-WPRE
Plasmid#166970PurposeCre-dependent viral expression of the human mu opioid receptor (hMOR/Oprm1) and mCherry. hMOR contains an N-terminal FLAG tag.DepositorInsertHomo sapiens opioid receptor mu 1 (OPRM1) (OPRM1 Human)
UseAAV and Cre/LoxTagsFLAG, T2A, and mCherryExpressionMammalianPromoterhsynAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2GG
Plasmid#203756PurposeEncodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-h-cGASCD(K427E/K428E)
Plasmid#127162Purposeexpression of truncated human cGAS proteinDepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS CoV-2 HexaPro spike (bio-His)
Plasmid#166856PurposeHuman expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.DepositorInsertSARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
Tags8xHis and Biotinylation siteExpressionMammalianAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
137_pAAV-ProA3-CatCh-GFP-WPRE
Plasmid#125887PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVPromoterProA3Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A
Plasmid#213527PurposeExpresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92ADepositorInsertNSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
TagsHis6-sumoExpressionBacterialMutationD90A and E92AAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_OC43_NSP14
Plasmid#213519PurposeExpresses NSP14 of hCoV OC43DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_NL63_NSP14
Plasmid#213520PurposeExpresses NSP14 of hCoV NL63DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_229E_NSP14
Plasmid#213521PurposeExpresses NSP14 of hCoV 229EDepositorInserthCoV 229E NSP14
TagsHis6-sumoExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-NES-ZapCV2 (cpV143)
Plasmid#112060PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorInsertNES-ZapCV2
TagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCMVAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only