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NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF

Plasmid

#163317

Purpose

Encodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferase

Depositor

Dawn Chandler

Insert

Nsp1, Nsp10, Nsp11 ,Nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Use

Luciferase

Tags

NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
nTRb412-gs10-NLS-VPR

Plasmid

#188267

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nTRb412 (TXNRD2 Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKalpha

Plasmid

#26208

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK alpha (CHUK Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/UUG-nLuc-3XFLAG

Plasmid

#127302

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1

Plasmid

#184566

Purpose

Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag A)

Plasmid

#16605

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag A) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragA (XmnI->KpnI). The KpnI site is at -1194 …

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSunS

Plasmid

#171672

Purpose

SunS expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

Plasmid

#162486

Purpose

For generating transgenic mice expressing ERK biosensor and RGECO1

Depositor

Michiyuki Matsuda

Article

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ERK biosensor based on FRET, and RGECO1

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJM160

Plasmid

#134169

Purpose

Bacterial expression of RB3 stathmin-like domain (SLD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

RB3-SLD (STMN4 Human)

Expression

Bacterial

Mutation

residues 49-189

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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TB:7xSMAD_RE:MiniP

Plasmid

#133879

Purpose

7 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies the SMAD DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Smpd4

Plasmid

#120237

Purpose

Expresses V5-tagged Smpd4 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Smpd4 (Smpd4 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pF208wt-luc

Plasmid

#122143

Purpose

For luciferase expression

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Fam208a promoter (Tasor Mouse)

Use

Luciferase

Promoter

Fam208a

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

Plasmid

#32728

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-947 AP-1 binding site TGAGTCA deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
POLArIS_NbVim-cp10GFP

Plasmid

#171020

Purpose

Expresses NbVim-cp10GFP#2 in mammalian cells

Depositor

Sumio Terada

Article

Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

Insert

anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)

Tags

cp10GFP

Expression

Bacterial and Mammalian

Promoter

CMV promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-MYB-3'UTR

Plasmid

#25798

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

3'UTR of human MYB (MYB Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-shLuc

Plasmid

#73666

Purpose

pSRIEN-RetroQ vector containing control sequence luciferase

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA to Luciferase

Use

Retroviral

Available Since

April 12, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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hGFAP R239C

Plasmid

#169476

Purpose

Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 239 to C

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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GADD45 P-2/3m-luc

Plasmid

#8359

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter P-2/3m (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

P-2/3m

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only