We narrowed to 14,501 results for: SHR

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FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

Plasmid

#234882

Purpose

L1HS-silencing plasmid (CRISPRi gRNA1)

Depositor

Johan Jakobsson

Article

Adami et al bioRxiv 2025.01.17.633315

Insert

L1HS-silencing plasmid (CRISPRi gRNA1)

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TUBA1B

Plasmid

#207763

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-U6ac-ctrl-guides

Plasmid

#168240

Purpose

"neutrophil specific GFP with ubiquitous ctrl sgRNAs"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

control sgRNAs

Use

CRISPR

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBA439

Plasmid

#85967

Purpose

Perturb-seq vector backbone

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-GFPg1 (BB09)

Plasmid

#139461

Purpose

Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

GFP-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-EN479

Plasmid

#86234

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

spCas9-nickase and sgRNA against mouse rosa26 locus

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pS548∙CsR

Plasmid

#197859

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Tc resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNT

Plasmid

#138681

Purpose

Expresses a non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-1

Plasmid

#83934

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCas-CmR(+)

Plasmid

#113633

Purpose

Chloramphenicol resistant derivative of pCas plasmid

Depositor

Olivier Lichtarge

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

Chloramphenicol resistance gene

Use

CRISPR

Promoter

Pcat

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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VCP F12.4 gRNA

Plasmid

#90939

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F12.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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VCP F11.4 gRNA

Plasmid

#90938

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F11.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GSK3β-#1

Plasmid

#32496

Depositor

Alex Toker

Article

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
pS648∙CsR

Plasmid

#197860

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-yA

Plasmid

#191015

Purpose

CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

Depositor

Perng-Kuang Chang

Article

Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

Insert

yA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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