178,769 results
-
Plasmid#79013PurposeFor mammalian expression of a shortened, constitutively active version of Tfeb.DepositorInsertTfeb (TFEB Human)
ExpressionMammalianMutationRemoved first ~400 bp from 5' end (N-terminu…PromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTubulin
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
ExpressionBacterialAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-FLAG-mCherry-C1-centrin-1
Plasmid#73333PurposeLentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tagsDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsFLAG and mCherryExpressionMammalianPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-mCherry-NES reporter (pDN160)
Plasmid#72660PurposeNLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-mCherry-NES
ExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDonor_hU6
Plasmid#69312PurposesgRNA scaffold and human U6 promoterDepositorInserthU6 promoter and sgRNA scaffold flanked by BbsI sites
UseCRISPRPromoterhuman U6 promoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-EF1-Luc2-P2A-copGFP
Plasmid#72485PurposeExpress Luc2 and copGFPDepositorInsertLuc2-P2A-copGFP
UseLentiviralExpressionMammalianPromoterEF-1Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET28a-MSP1D1deltaH4-H6
Plasmid#71717PurposeMembrane scaffold protein (MSP), helix 4 to 6 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
ExpressionBacterialAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-5ptaseOCRL
Plasmid#66836PurposeExpression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5ptaseOCRL (OCRL Human)
TagsmCherry-CRY2ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-HA-Nrxn1beta AS4(-)
Plasmid#59409PurposeExpression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorPublicationAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-Jaws-KGC-GFP-ER2
Plasmid#65014PurposeAAV-mediated expression of Jaws under the human synapsin promoterDepositorHas serviceAAV5, AAV8, and AAV RetrogradeInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterhuman synapsinAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PGK-AAVS1ZFNL
Plasmid#60916Purposeencodes zinc finger nuclease specific to AAVS1 locus (left)DepositorPublicationInsertAAVS1-ZFNL
PromoterPGKAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJET1.2-STOP-dsRed
Plasmid#60944PurposeDrosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.DepositorInsertdsRED
UseUnspecifiedPromoter3XP3Available sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1
Plasmid#58434PurposeInduces Citrine expression from a promoter containing 4 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)4-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cerulean-N1
Plasmid#54742PurposeLocalization: N1 Cloning Vector, Excitation: 433, Emission: 475DepositorTypeEmpty backboneTagsCeruleanExpressionMammalianAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN HAYFP-LOV24
Plasmid#49570Purposeregulate protein stability with lightDepositorInsertYFP-LOV24
UseRetroviralTagsHA and YFPMutationdelete the last 3 aa from AsLOV2, with I532A muta…PromoterLTRAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GABA(A) receptor subunit a2SE
Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
TagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EMM65
Plasmid#49042PurposeBackbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2DepositorTypeEmpty backboneUseTale lsd1 expression vectorTagsLSD1ExpressionMammalianPromoterEF1 alphaAvailable sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by