We narrowed to 6,529 results for: nls
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Plasmid#107304PurposeExpresses ZFP-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertZFP_VEGFA-TS3
UseCRISPRTags2xNES, 3x Flag, 2xNLS, and FRBExpressionMammalianPromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta2
Plasmid#100871PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
Tags3xFLAGExpressionMammalianMutationtruncated protein 71-246aaPromoterCMVAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Flag-NLS-hSPDEF delta3
Plasmid#100876PurposeLentiviral expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationtruncated protein 243-335aaPromoterTRE2Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta3
Plasmid#100872PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
Tags3xFLAGExpressionMammalianMutationtruncated protein 244-335aaPromoterCMVAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1-Flag-NLS-hSPDEF delta1
Plasmid#100870PurposeMammalian expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
Tags3xFLAGExpressionMammalianMutationtruncated protein 1-248aaPromoterCMVAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
TagsmCherryExpressionYeastPromoterRPS2Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-CHES1L-DBD-NLS
Plasmid#51841PurposeFlag-CHES1L mutant encoding the DNA binding and NLS domains onlyDepositorInsertCHES1 (FOXN3 Human)
UseRetroviralTagsFlag and NLSMutationcontains CHES1 amino acids 110-205Available SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
SPL11p::pCGTAG (SPL11p::NLS-GFP)
Plasmid#27411DepositorInsertSPL11 promoter (AT1G27360 Mustard Weed)
MutationSPL11 promoter transcriptionally fused to a nucle…Available SinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)
Plasmid#115141PurposeCMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLSDepositorInserthuman codon optimized Orf2mor anti-CRISPR protein
TagsNLS(SV40)ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry
Plasmid#68280PurposeSKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherryDepositorAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMVd1-NLS-GAL4-iLight2-VP16
Plasmid#219550PurposeNLS-Gal4(1-65)-iLight2-VP16 construc expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight2-VP16
UseAAVExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1Available SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Catalytically Enhanced (E655G and N696I) -ThermoCas9-3xNLS
Plasmid#254684PurposeExpresses Catalytically Enhanced (E655G and N696I) ThermoCas9 with 3x NLS tags. Utilized for RNP transfectionDepositorInsertCatalytically Enhanced (E655G and N696I) ThermoCas9
TagsNLS, SV40 NLS, and nucleoplasmin NLSExpressionBacterialAvailable SinceMay 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA
Plasmid#118617PurposeLentiviral expression of mTurq2 tagged PCNADepositorInsertTK-NLS-mTurq2-PCNA (PCNA Human)
UseLentiviralTagsmTurq2ExpressionMammalianPromoterhPGKAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
tetO-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69545PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.DepositorInserteGFP
UseLentiviralTags2x nuclear localization sequence and T2A "sk…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-5'UTR-bpNLS-BxbI-3'UTR-polyA (CJT476)
Plasmid#248216PurposebpNLS-BxbI IVT vector for mRNA productionDepositorInsert5'UTR-bpNLS-BxbI-3'UTR-polyA
UseCRISPR; In vitro transcriptionTagsBPNLSExpressionMammalianMutationNonePromoterT7Available SinceMay 12, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSTV6-3xFLAG-miniTurbo-NLS-VHL54:213
Plasmid#248673PurposeTo generate cells expressing Nterm tagged miniTurbo deltaVHL for ProtacID via lentivirus.DepositorAvailable SinceMarch 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1a-dCas9-HA-BFP-KRAB-NLS
Plasmid#102244PurposeLentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoterDepositorInsertdCas9-HA-BFP-KRAB-NLS
UseCRISPR and LentiviralTagsHA-TagBFP-KRAB-NLSExpressionMammalianPromoterEF1aAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-Pest
Plasmid#240116PurposeA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.DepositorInsertmurine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded SV40-EnhancerAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3
Plasmid#170175PurposeCation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2f-ST-P2A-NLS-mRuby3
UseAAVExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
Plasmid#31476DepositorInsertSFFV d14 GFP donor EF1s I-SceI
UseLentiviralTagsHA and NLSMutationGFP has 14 amino acids deleted from c terminusAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-mScaI3-NLS-Pest
Plasmid#240107PurposeA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorInsertmurine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianPromoterEF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-Venus-NLS-Pest
Plasmid#240106PurposeA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorInsertmurine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest
Plasmid#240112PurposeA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.DepositorInsertmurine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded SV40-EnhancerAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)
Plasmid#225754PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS-FLAG-cGAS
Plasmid#127659PurposeOver-express EGFP-NLS-FLAG-cGASDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 R62D actin-3XNLS P2A mCherry
Plasmid#58477PurposeExpresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorInsertsTagsmCherryExpressionMammalianMutationChanged Arginine 62 to Aspartic AcidPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
Plasmid#193968PurposeEncodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoterDepositorAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
Plasmid#34911DepositorInsertpresynaptic mGRASP
UseAAVPromoterCAGAvailable SinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc
Plasmid#113899PurposeMammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminaseDepositorInsertTetR-HA-NLS-P2A-BSD-Myc
UseLentiviralTagsHA-MycPromoterCMV-MIEAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato
Plasmid#51084PurposeNeuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV Retrograde and AAV1InsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianPromoterhuman Synapsin1Available SinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR myc-MBP-SspB-iRFP-NLS
Plasmid#223286PurposeAn SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.DepositorInsertMyc-MBP-SspB-irFP-NLS
UseLentiviralExpressionMammalianAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3
Plasmid#73205PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertNLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
TagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)
Plasmid#140534PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2DepositorInsertOsTIR1(F74G) mAID-EGFP-NLS TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-MCS-MED1long-IDR-YFP-NLS
Plasmid#145276PurposeMammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLSDepositorInsertMED1-IDR long (MED1 Human)
ExpressionMammalianAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-NLS-mVenus
Plasmid#118619PurposeLentiviral expression of the cell cycle marker PIP-NLS-mVenusDepositorInsertPIP-NLS-mVenus
UseLentiviralTagsmVenusExpressionMammalianPromoterhPGKAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
Plasmid#132543PurposeExpresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cellsDepositorInsertCIRTS-1: ORF5-TBP6.7-Pin domain-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only