Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 1481 - 1520 of 18759 results

  6. CMV Marina-T2A-nls-mCherry

    Plasmid
    #74216
    Purpose
    genetically-encoded fluorescent voltage indicator
    Depositor
    Vincent Pieribone
    Publication
    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    fluorescent protein voltage sensor
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    CMV
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74216-plasmid-map-sequence-id-170988

    Enlarge

  7. pCMV-dLbCpf1-BE-YE1

    Plasmid
    #154145
    Purpose
    Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.
    Depositor
    Jin-Soo Kim
    Publication
    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-BE-YE1 (dCas12a-BE-YE1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)
    Promoter
    CMV
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    154145-plasmid-map-sequence-id-306832

    Enlarge

  15. pRD400

    Plasmid
    #163107
    Purpose
    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_eYFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163107-plasmid-map-sequence-id-321848

    Enlarge

  20. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199375-plasmid-map-sequence-id-391013

    Enlarge

  21. pRD405

    Plasmid
    #163110
    Purpose
    Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS_mScarlet-I_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163110-plasmid-map-sequence-id-321851

    Enlarge

  22. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

    Enlarge

  23. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid
    #92354
    Purpose
    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92354-plasmid-map-sequence-id-197504

    Enlarge

  26. phage UbiC NLS HA stdPCP stdGFP

    Plasmid
    #104099
    Purpose
    Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.
    Depositor
    Jeffrey Chao
    Publication
    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d
    Insert
    stdPCP-stdGFP
    Use
    Lentiviral
    Tags
    N-terminal NLS, Two synonymous copies of GFP (C-t…
    Expression
    Mammalian
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104099-plasmid-map-sequence-id-200989

    Enlarge

  32. GFP-polyQ74-NLS

    Plasmid
    #231892
    Purpose
    Forms nuclear HTT partial exon 1 Q74-NLS aggregates
    Depositor
    Lukas Kapitein
    Publication
    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT partial exon 1 Q74-NLS (HTT Human)
    Tags
    EGFP and SV40 NLS
    Expression
    Mammalian
    Mutation
    CAG repeat expansion
    Available since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    231892-plasmid-map-sequence-id-461348

    Enlarge

  33. pBT3047

    Plasmid
    #122547
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a N-terminal (sv-40) and C-terminal (egl-13) nuclear localization signal under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_NLS(sv-40)::CemOrange2::NLS(egl-13)
    Tags
    MAPKKKRKVV and MSRRRKANPTKLSENAKKLAKEVEN
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122547-plasmid-map-sequence-id-238211

    Enlarge

  35. pcDNA3-eGFP-NLS-RNF168

    Plasmid
    #133977
    Purpose
    mammalian expression vector of eGFP tagged wild type RNF168
    Depositor
    Daniel Durocher
    Publication
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNF168 (RNF168 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA
    Promoter
    CMV
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133977-plasmid-map-sequence-id-261816

    Enlarge

  39. pCS2+ GCamp6-3NLS

    Plasmid
    #165401
    Purpose
    Expresses a nuclear localized genetically encoded calcium reporter.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Localization Signal
    Use
    Avian, xenopus, zebrafish
    Tags
    GCamp6
    Expression
    Mammalian
    Promoter
    SP6
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165401-plasmid-map-sequence-id-325983

    Enlarge

Showing: 1481 - 1520 of 18759 results