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We narrowed to 6,273 results for: nls

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Showing: 1481 - 1520 of 6273 results

  1. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  2. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51086
    Purpose
    Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Promoter
    CaMKIIa 1.3 kb
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51086-plasmid-map-sequence-id-469612

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  7. pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-Pest

    Plasmid
    #240116
    Purpose
    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added SV40-Enhancer
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240116-plasmid-map-sequence-id-481764

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  15. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  18. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid
    #89993
    Purpose
    Donor template for generation of SOX9-ntdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    Use
    CRISPR
    Promoter
    none
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    89993-plasmid-map-sequence-id-180698

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  22. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

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  30. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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  34. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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  37. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Article
    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131132-plasmid-map-sequence-id-255500

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  38. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid
    #177345
    Purpose
    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    dead hSaCas9
    Use
    AAV and CRISPR
    Tags
    3x GCN4 peptide, 3xHA, and NLS
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    Synapsin
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177345-plasmid-map-sequence-id-353619

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Showing: 1481 - 1520 of 6273 results