Addgene

We narrowed to 5,865 results for: nls

Showing: 1481 - 1520 of 5865 results

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F3

Plasmid

#236448

Purpose

insect expression of E2F3 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3 (E2f3 Mouse)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F1

Plasmid

#236446

Purpose

insect expression of E2F1 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F2

Plasmid

#236447

Purpose

insect expression of E2F2 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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UAST-FRT-GFP-FRT-uMCas12a+nls1x

Plasmid

#230908

Purpose

Double inducible expression of Cas12a+ with Gal4 and Flp.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

Cas12a+

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-KanMX6

Plasmid

#228022

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p-TID-FRB-NLS-HA3

Plasmid

#228009

Purpose

Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p- TID-FKBP-NLS-HA3

Plasmid

#228011

Purpose

Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CHA1p-TID-FRB-NLS-HA3

Plasmid

#228013

Purpose

Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

CHA1

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CHA1p- TID-FKBP-NLS-HA3

Plasmid

#228015

Purpose

Expresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

CHA1

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-NLS-3HA-HIS3MX6

Plasmid

#228023

Purpose

To integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FKBP-NLS-3HA-HISMX6

Plasmid

#228027

Purpose

To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with HISMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BirA-FRB-NLS-HA3

Plasmid

#227985

Purpose

Expresses BirA-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BirA-FKBP-NLS-HA3

Plasmid

#227987

Purpose

Expresses BirA-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BioID2-FRB-NLS-HA3

Plasmid

#227989

Purpose

Expresses BioID2-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p- BioID2-FKBP-NLS-HA3

Plasmid

#227991

Purpose

Expresses BioID2-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p- BASU-FRB-NLS-HA3

Plasmid

#227993

Purpose

Expresses BASU-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p- BASU-FKBP-NLS-HA3

Plasmid

#227995

Purpose

Expresses BASU-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FRB-NLS-HA3

Plasmid

#227997

Purpose

Expresses TurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FKBP-NLS-HA3

Plasmid

#227999

Purpose

Expresses TurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-mTID-FRB-NLS-HA3

Plasmid

#228001

Purpose

Expresses miniTurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-mTID-FKBP-NLS-HA3

Plasmid

#228003

Purpose

Expresses miniTurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-HIS3p-TID-FRB-NLS-HA3

Plasmid

#228005

Purpose

Expresses TurboID-FRB-NLS fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p- TID-FKBP-NLS-HA3

Plasmid

#228007

Purpose

Expresses TurboID-FKBP-NLS fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-MET25p-TID-FRB-NLS-HA3

Plasmid

#228017

Purpose

Expresses TurboID-FRB-NLS fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-MET25p- TID-FKBP-NLS-HA3

Plasmid

#228019

Purpose

Expresses TurboID-FKBP-NLS fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFa6-TID-FKBP-NLS-3HA-KanMX6

Plasmid

#228026

Purpose

To integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552-DIO-NLS-mRuby3(with gRNA scaffold)

Plasmid

#199583

Purpose

Expresses NLS-mRuby3 in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

NLS-mRuby3

Use

AAV, CRISPR, and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

U6 and EF1a

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP-Cre (JDW 1329)

Plasmid

#224515

Purpose

A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

nls-EGFP-Cre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

Plasmid

#177344

Purpose

AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

dead hSaCas9

Use

AAV and CRISPR

Tags

3x GCN4 peptide, 3xHA, and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

Tetracycline-dependent promoters

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

Plasmid

#177342

Purpose

AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

dead hSaCas9

Use

AAV and CRISPR

Tags

3x GCN4 peptide, 3xHA, and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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L0-T13 GG3-tRBCS-mCherry-NLS-GG4

Plasmid

#219696

Purpose

Level 0 to construct history-dependent target

Depositor

Jennifer Nemhauser

Insert

GG3-tRBCS-mCherry-NLS-GG4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1

Plasmid

#219697

Purpose

Level 0 to construct history-dependent target

Depositor

Jennifer Nemhauser

Insert

GG0-tUBQ1-NLS-NeonGreen-GG1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMVd1-NLS-GAL4-iLight-VP16

Plasmid

#219549

Purpose

NLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight-VP16

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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SOX2-P2A-NLS-mClover3-IRES-Puro

Plasmid

#215544

Purpose

Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

Depositor

Kai Wang

Insert

SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

Plasmid

#212737

Purpose

Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT mCh-V5-NES-EGFP-EGFR NLS

Plasmid

#192315

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT mCh-V5-NES-EGFP-ERBB2 NLS

Plasmid

#192316

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS

Plasmid

#192317

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT mCh-V5-NES-EGFP-SV40 NLS

Plasmid

#192318

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-SV40 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-SV40 NLS

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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