159,589 results

1. pEXP(V5-APEX2-NES)

   Plasmid

   #107596

   Purpose

   Expresses V5-APEX2-NES in mammalian cells

   Depositor

   Markus Hafner

   Article

   Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

   Insert

   APEX2-NES
   

   Use

   Frt site to insert into hek293 t-rex genome

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Dox inducible

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-PIP5K1A

   Plasmid

   #202720

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   EGFP:PIP5K1A (PIP5K1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-tetO-hOKMS

   Plasmid

   #51543

   Purpose

   Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

   Depositor

   Tarjei Mikkelsen

   Article

   Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

   Insert

   hOKMS (MYC, KLF4, SOX2, POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet_peroxisome_C1

   Plasmid

   #85063

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Peroxisomal targeting sequence
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

   Plasmid

   #105556

   Purpose

   AAV expression of EGFP from tMCK promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tMCK

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV PIG (Puro IRES GFP empty vector)

   Plasmid

   #21654

   Purpose

   Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus-pBAD

   Plasmid

   #54859

   Purpose

   Localization: Protein Expression Vector , Excitation: 515, Emission: 528

   Depositor

   Atsushi Miyawaki, Michael Davidson

   Article

   Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

   Type

   Empty backbone

   Use

   Tags

   6xHis and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. RMCE K>R DNA-PKcs

   Plasmid

   #233917

   Purpose

   Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K3753R

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    flag-halo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Sport6-CD63-pHluorin

    Plasmid

    #130901

    Purpose

    Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD63-pHluorin (CD63 Human, Aequorea victoria)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    pHluorin inserted between Gln-36 and Leu-37 in ex…

    Promoter

    CMV

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSP64T (DM#111)

    Plasmid

    #15030

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFPmAID

    Plasmid

    #174213

    Purpose

    GFP::mAID donor backbone

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx4-Cdc42-2G

    Plasmid

    #68814

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

    Plasmid

    #130999

    Purpose

    Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet-Kv2.1

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307-TMPRSS2-puro

    Plasmid

    #158457

    Purpose

    Constitutive lentiviral expression of human TMPRSS2.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCRISPRa_VPR_yl

    Plasmid

    #107677

    Purpose

    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

    Inserts

    Codon optimized dCas9-VPR
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AA0250-mCherry-2a-hM4D-nrxn1a rev

    Plasmid

    #60544

    Purpose

    Co-expresses hM4Dnrxn and a red fluorescent label from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Inserts

    mCherry
    muscarinic receptor 4, axon targeted variant (CHRM4 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3XMEF2-luc

    Plasmid

    #32967

    Purpose

    Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

    Depositor

    Ron Prywes

    Insert

    MEF2 binding sites (MEF2A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    MEF2

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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