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pLG001 (empty vector)

Plasmid

#157879

Purpose

Empty vector backbone for defense systems

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

None

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.tdTomato.WPRE.SV40

Plasmid

#105554

Purpose

AAV expression of tdTomato from CAG promoter

Depositor

James M. Wilson

Insert

tdtomato

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-eGFP-P2A-dTAG-Ing3

Plasmid

#170754

Purpose

Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

eGFP-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

eGFP-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

none

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMK1254

Plasmid

#133057

Purpose

Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB1

Plasmid

#200981

Purpose

Mammalian expression plasmid for myc-tagged EphB1

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB1 (EPHB1 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pORI28

Plasmid

#71595

Purpose

EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Type

Empty backbone

Use

Integration vector for bacteria

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-MYC-t2A-BCL2

Plasmid

#135306

Purpose

Overexpression of multiple human genes (BCL2 and MYC)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

MYC (MYC Human)
BCL2 (BCL2 Human)

Use

Retroviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

Plasmid

#112926

Purpose

CRISPR-a reporter assay lentiviral vector (empty gRNA control)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(empty), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-mDia1-N-14

Plasmid

#54157

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

mDia1 (DIAPH1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAcGP67A - murine TfR

Plasmid

#12392

Depositor

Pamela Bjorkman

Insert

transferrin receptor (Tfrc Mouse)

Tags

6X-His tag, Factor Xa cleavage site, and leader p…

Expression

Insect

Mutation

See comments section

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pUC19-EAMP

Plasmid

#138265

Purpose

mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

iCas9 target-mCherry

Use

CRISPR

Expression

Mammalian

Promoter

EF1a-HTLV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B

Plasmid

#121157

Purpose

Mammalian expression of GFP tagged TIS11B

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) (ZFP36L1 Human)

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc2

Plasmid

#74050

Purpose

retroviral expression plasmid for human NFATc2/C

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc2, isoform C (NFATC2 Human)

Use

Retroviral

Tags

no tag

Expression

Mammalian

Mutation

silent mutation A1723C in the sequence of NM_1730…

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7s

Plasmid

#104463

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7s

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR APLP1 shRNA 2

Plasmid

#30157

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP1 shRNA 2

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Mutation

GFP has 20 amino acids deleted from the C terminu…

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCK054 pDEST exorh:mCerulean

Plasmid

#195985

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-Luciferase

Plasmid

#21375

Purpose

self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

Depositor

Bryan Welm

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pABY-c45

Plasmid

#201771

Purpose

Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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PGK-DTAG-NTERM-GFP

Plasmid

#185775

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

PGK

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5a-7

Plasmid

#55126

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Rab5a

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-GST-Rnf31

Plasmid

#63134

Purpose

Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

Rnf31 (RNF31 Human, Synthetic)

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
Anti-CASK [K56A/50R]

Plasmid

#177443

Purpose

Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/50.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Epsilon [N413/67R]

Plasmid

#114478

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Epsilon (Human). Derived from hybridoma N413/67.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Epsilon (Homo sapiens) recombinant mouse monoclonal antibody (GABRE Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF1683

Plasmid

#142826

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TWIST1 (TWIST1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3165

Plasmid

#144641

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TRIM28 (TRIM28 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2R

Plasmid

#167932

Purpose

lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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