We narrowed to 14,009 results for: flag

1. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

   Plasmid

   #101062

   Purpose

   AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   CaMPARI2-H396K
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Bxb1Donor_WT_ADRB2_LucReporter

   Plasmid

   #129788

   Purpose

   encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Synthetic Biology

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available Since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDB-hCRY1-mClover3-CD4-bla

   Plasmid

   #179441

   Purpose

   Donor vector to knock in mClover3 C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mClover3
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAcBac1-Mb-Fluoro-Phe B5

   Plasmid

   #197576

   Purpose

   Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

   Inserts

   Mb Pyl Fluoro-Phe B5 synthetase
   Pyl-tRNA (4 copies)
   

   Use

   Tags

   Nuclear export sequence + FLAG

   Expression

   Mammalian

   Mutation

   N311G, C313A

   Promoter

   CMV and U6/H1

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pL40C_PGKintron_Cas9_Green

   Plasmid

   #134966

   Purpose

   Lentiviral vector coding Cas9 and mNeonGreen

   Depositor

   Beat Bornhauser

   Article

   Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

   Insert

   Cas9-P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK promoter with beta-globin intron

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMSCV-MAVS(IRSp53 pLxIS)

   Plasmid

   #221315

   Purpose

   Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR1A mKate2-hu_ncOGT D554N

   Plasmid

   #154283

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Expression

   Mutation

   D554N mutation (Ser/Thr Glycosylation Impaired)

   Promoter

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gal4-entry+NLS

   Plasmid

   #128013

   Purpose

   Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   Gal4 DBD-SV40NLS-3x-Flag
   

   Use

   Gal4 entry vector with nls to generate tethering …

   Tags

   Gal4-DBD-SV40NLS-3xFlag

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB-hCRY1-Halo-CD4-bla

   Plasmid

   #179445

   Purpose

   Donor vector to knock in Halotag C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   HaloTag (Halo7 Synthetic)
   

   Use

   CRISPR

   Tags

   His/Flag

   Expression

   Mutation

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA 3.1 KBTBD4 R312RG

    Plasmid

    #184630

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R312RG

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid

    #154285

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations (HCF-…

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac SpyTag-β2AR-SpyTag

    Plasmid

    #135620

    Purpose

    Expression of SpyTag-B2AR in insect cell

    Depositor

    Hee-Jung Choi

    Article

    Choi et al Science. 2019 Nov 29;366(6469):1150-1156

    Insert

    Beta 2 adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

    Expression

    Insect

    Mutation

    Deleted 1-24 aa, 366-413 aa

    Promoter

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pRK-ATF4 1-275

    Plasmid

    #26118

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 276-351 have been deleted

    Promoter

    Available Since

    March 1, 2011

    Availability

    Academic Institutions and Nonprofits only

15. U6-hGRIN2B-CAG-ps-SaCas9

    Plasmid

    #102853

    Purpose

    A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter;

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTBL469

    Plasmid

    #126458

    Purpose

    Expresses Cas9-15xFlag-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    Cas9
    15x Flag
    TdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST40-CES2A-ΔC-S227A

    Plasmid

    #200556

    Purpose

    Mammalian expression of secreted mouse CES2A S227A mutant

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
    

    Use

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST40-CES2A-ΔC

    Plasmid

    #200554

    Purpose

    Mammalian expression of secreted mouse CES2A

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
    

    Use

    Tags

    6xHis, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-Tbg-CES2A-ΔC

    Plasmid

    #200558

    Purpose

    Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    TBG promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK-ATF4 111-351

    Plasmid

    #26119

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa's 1-110 have been deleted

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pRK-ATF4 248-351

    Plasmid

    #26120

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ATF4 aa's 1-247 have been deleted

    Promoter

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pTF-ACE2s

    Plasmid

    #162786

    Purpose

    The extracellular domain of Angiotensin converting enzyme 2 (ACE2) expression

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    10xHis, FLAG, and hTPA leader

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid

    #24419

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    RS1 (HTR4 Human)
    

    Use

    Mouse Targeting

    Tags

    FLAG and mCherry

    Expression

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Promoter

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV10-mFoxP1-ES

    Plasmid

    #35169

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

28. pGEX6p-1-OSF-UFM1

    Plasmid

    #204565

    Purpose

    Expresses GST-Strep-FLAG-tagged UFM1 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFM1 (UFM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    delta84-85aa

    Promoter

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. B3GALT5_pLENTI-CAG-IRES-GFP

    Plasmid

    #177006

    Purpose

    Mammalian lentiviral expression vector encoding B3GALT5

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    B3GALT5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDB-hPER2-Halo-CD4-bla

    Plasmid

    #179451

    Purpose

    Donor vector to knock in Halotag C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    HaloTag (Halo7 Synthetic)
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge