19,660 results

1. pGFPmAID

   Plasmid

   #174213

   Purpose

   GFP::mAID donor backbone

   Depositor

   Masato Kanemaki

   Article

   Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-3c-rhVTN-N

   Plasmid

   #30225

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N terminus cleavage (VTN Human)
   

   Expression

   Bacterial

   Mutation

   deleted amino acids: 20*-62

   Available Since

   May 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SUPERBLADE5

   Plasmid

   #134913

   Purpose

   Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

   Depositor

   Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Promoter

   U6

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCI-neo Fluc EGFP

   Plasmid

   #90170

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-GFP-MT2A

   Plasmid

   #11613

   Depositor

   Steven Johnson

   Insert

   metallothionein 2A (MT2A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EGFP-Rab11a-7

   Plasmid

   #56444

   Purpose

   Localization: Ras GTPase, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

   Insert

   Rab11a (RAB11A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pWZL hygro 12S E1A

   Plasmid

   #18748

   Depositor

   Scott Lowe

   Article

   Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

   Insert

   12S E1A
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDH-puro-Bcl2

   Plasmid

   #46971

   Purpose

   lentiviral expression of Bcl2

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   Bcl2 (BCL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSDest2

   Plasmid

   #22424

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Type

   Empty backbone

   Use

   Destination vector

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mApple-TOMM20-N-10

    Plasmid

    #54955

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p45sheLL

    Plasmid

    #37323

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV45L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.3_MERS_D12

    Plasmid

    #170448

    Purpose

    Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    MERS spike D12 (S MERS)
    

    Expression

    Mammalian

    Mutation

    last 12aa deletion in c-terminal tail

    Promoter

    CMV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCF806-reci

    Plasmid

    #186711

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. phage UbiC NLS HA stdMCP stdHalo

    Plasmid

    #104999

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    stdMCP-stdHalo
    

    Use

    Lentiviral

    Tags

    HA tag, NLS, Two synonymous copies of the Halo-ta…

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGCC4

    Plasmid

    #37058

    Depositor

    Hank Seifert

    Type

    Empty backbone

    Available Since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p-EGFP-C1-Flag-mFmr1(wt)

    Plasmid

    #87929

    Purpose

    Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

    Depositor

    Stephanie Ceman

    Article

    Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-EGFP-HaloTag2

    Plasmid

    #41742

    Depositor

    Craig Crews

    Article

    Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

    Insert

    Halotag 2
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Str-Ii_SBP-mCherry-Golgin84

    Plasmid

    #65304

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121184

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    CMV-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Mutation

    OsTIR1 codon optimized for human cells

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge