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Showing: 7721 - 7740 of 9090 results

  2. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid
    #232929
    Purpose
    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    OsTir1(F74G)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    changed Phenylalanine 74 to Glycine
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232929-plasmid-map-sequence-id-466497

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  6. pScRad52-SpCas9 (pXK-1646)

    Plasmid
    #208823
    Purpose
    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Use
    CRISPR; Cas9
    Tags
    Rad52
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    208823-plasmid-map-sequence-id-417236

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  7. pDC-UAS-nBitbow1.0(miniW)

    Plasmid
    #138329
    Purpose
    Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.
    Depositor
    Dawen Cai
    Article
    Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3
    Inserts
    mAmetrine
    tdKatushka2
    mNeonGreen
    mTFP1
    mKusabira-Orange2
    Tags
    human H2B
    Expression
    Insect
    Promoter
    10xUAS
    Available Since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138329-plasmid-map-sequence-id-275089

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  8. AWP-029

    Plasmid
    #213966
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Segatella bryantii, Synthetic)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213966-plasmid-map-sequence-id-429164

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  13. pGA-red-maxi

    Plasmid
    #196337
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196337-plasmid-map-sequence-id-393160

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  15. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid
    #218514
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(1)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218514-plasmid-map-sequence-id-444100

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  17. pAW212

    Plasmid
    #172196
    Purpose
    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sites
    Depositor
    Chang Liu
    Article
    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038
    Insert
    p10B2-AT110
    Expression
    Yeast
    Promoter
    10B2 (P1 specific promoter)
    Available Since
    Aug. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    172196-plasmid-map-sequence-id-338565

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  19. pEvolvR-mCherry-enCas9(H840A)-PolI5M

    Plasmid
    #249064
    Purpose
    Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9(H840A)-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249064-plasmid-map-sequence-id-495619

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  20. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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Showing: 7721 - 7740 of 9090 results