We narrowed to 8,596 results for: Set

1. pLV-Hygro-EF1A-OB-Linker-eGFP

   Plasmid

   #176068

   Purpose

   EGFP fused to the C-terminus of an OB domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   OB
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLV-Hygro-EF1A-FHA-Linker-eGFP

   Plasmid

   #176065

   Purpose

   EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   FHA
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE

   Plasmid

   #196414

   Purpose

   Astrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smFLAG
   

   Use

   AAV; Astrocyte-selective

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-GFP-MCC

   Plasmid

   #133307

   Purpose

   "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   microcin-V bacteriocin cassette (cvaC E. coli)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N112D (please see depositors comments)

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19_Ntag_BbsI-Mammalian

   Plasmid

   #186674

   Purpose

   N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Mammalian N-terminal Tag
   

   Tags

   3xFlag-3xHA

   Expression

   Mammalian

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Hygro-EF1A-Macro-Linker-eGFP

   Plasmid

   #176067

   Purpose

   EGFP fused to the C-terminus of a Macrodomain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Macro
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Hygro-EF1A-WWE-Linker-eGFP

   Plasmid

   #176072

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   WWE
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-GfaABC1D-iCreV-4x6T

   Plasmid

   #196412

   Purpose

   Astrocytic expression of light-inducible iCreV recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   iCreV
   

   Use

   AAV; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Hygro-EF1A-PBZC-Linker-eGFP

    Plasmid

    #176070

    Purpose

    EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PBZ-C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Hygro-EF1A-PBM-Linker-eGFP

    Plasmid

    #176069

    Purpose

    EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PBM
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid

    #191334

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid

    #139086

    Purpose

    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTMiniTrex-mCherry-Shark2

    Plasmid

    #233144

    Purpose

    Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark2
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Mutation

    Codon optimized for Trypanosoma cruzi

    Available Since

    March 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLC-HA active Nedd8-P2A-Hygro

    Plasmid

    #124307

    Purpose

    Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    Nedd8 (NEDD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Five C-terminal amino acids missing compared to w…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-PdTK-CAG-mCh-[uBglII]

    Plasmid

    #107280

    Purpose

    puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
    

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2048

    Plasmid

    #143332

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM9 (PRDM9 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_ASCL1

    Plasmid

    #64129

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AA145

    Plasmid

    #215937

    Purpose

    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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